Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Hydra vulgaris
    • Sequence

      MQYKLALNGK TLKGETTTEA VDAATAEKVF KQYANDNGVD GEWTYDDATK TFTVTELVPR GSQLWMALGT QTSAIESRPR SSINKNLCRA LYLHHYQRTV CLNYTDLMLS VAEGIRLGID ECQVQFKHRK WNCTINEHGT SVFGPIITTA SRESAFISGI ISAGVAFSVT ESCAEGKSVH CRCDNSVRGQ TDEGWRWGGC NRPITYGIWF SQLFIDQVEK IVKKRKDPRK IMNLHNNKAG REVIKNLLQT ECKCHGTSGN CNLKTCWRSQ PHFSEIGKIL KEKYDSAHEM EFLYKVKANG ERKIKDLIPK YKEYLPPSSL DFIYYEESPN YCVKNETLGI AGTKGRSCNI TSSGVDGCEL MCCQRGYNVN IVQKTHSCEC KFVWCCKVSC NSCIKMTPEY TCKLVPRGSL EHHHHHH
    • Molecular weight

      47 kDa
    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab136932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity

    > 95 % Purified via His tag.

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 9.50
    Constituents: 0.078% Beta mercaptoethanol, 6.9% DL-Arginine

General Info

  • Alternative names

    • Protein Wnt 3a Precursor
    • Protein Wnt-3a
    • Wingless type MMTV integration site family member 3A
    • Wnt 3a
    • wnt3a
    • WNT3A protein
    • WNT3A_HUMAN
    see all
  • Function

    Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
  • Tissue specificity

    Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.
  • Sequence similarities

    Belongs to the Wnt family.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

Images

  • 30 µl of 10% SDS (with betaME) was taken with 30 µl of cell pellet after 3 and 4 hours of induction with IPTG. The mixture was incubated for 5 mins at 95°C and 15 µl was loaded on the gel. Lanes 5, 6 and 7 shows presence of ab136932 Wnt3a protein detected by anti-Histidine antibodies.

References

ab136932 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us.

I have obtained the following information for you:

For ab136932: This protein is of invertebrate origin, specifically the cnidarian, Hydra. Thus, it is neither of mouse nor human origin. We will add this important information to the datasheet shortly.

For ab81484: The supplying lab has only limited supplies of this protein and would like to know how much protein would you require?

I look forward to hear back from you.

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Abreviews
Application
Cellular Activation
Material tested at 400, 200, and 100ng/ml alongside R&D Systems mWnt3a protein (100ng/ml). No activity in CellSensor assay (Life Technologies) from Abcam mWnt3a (ab136932material.

Dr. Paul Weingarten

Verified customer

Submitted Jan 08 2014

Question
Answer

Thank you for contacting us and your interest in our products. I am sorry for the delay in getting back to you. I now have further information to share.

As discussed over the phone, unfortunately we are currently experiencing some problems in the production of the Wnt3 protein (ab81484) and we are unlikely to have it in stock for the foreseeable future. We have suggested to try ab136932 as an alternative. However, we do not unfortunately have any information as to whether this is likely to perform in functional assays. It may be that the protein has biological acitivty, but as thishas not been tested we cannot guarantee it.

What I would be able to do is offer you a testing discount. This offer means that if you purchase ab136932 as normal, test theprotein in your functional assayand let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for the equivalent price of ab136932 off any product in our catalogue. More information on this offer can be found here:

www.abcam.com/collaborationdiscount

Please note that theproduct to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab136932.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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