Product nameRecombinant Hepatitis B Surface Antigen (Adw) protein
Expression systemPichia pastoris
Protein lengthFull length protein
Predicted molecular weight24 kDa
Amino acids56 to 281
Additional sequence informationThe protein contains immunodominant region of HBV surface antigen adw1 subtype.
Our Abpromise guarantee covers the use of ab91276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab91276 contains the Hepatitis B Virus Surface Antigen immunodominant region.
Purity is 50-60 % determined by SEC-HPLC.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze.
Constituents: 0.58% Sodium chloride, PBS, 0.21% Sodium carbonate, 0.09% EDTA, 15% Glycerol
- S gene subtype adw2
RelevanceHepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.
Cellular localizationMembrane Virion
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab91276 has been referenced in 2 publications.
- Martinez DR et al. Fc Characteristics Mediate Selective Placental Transfer of IgG in HIV-Infected Women. Cell 178:190-201.e11 (2019). PubMed: 31204101
- Thomsen MK et al. Lack of immunological DNA sensing in hepatocytes facilitates hepatitis B virus infection. Hepatology 64:746-59 (2016). PubMed: 27312012