Key features and details
- Expression system: Escherichia coli
- Purity: > 90% Densitometry
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, WB, ELISA
Product nameRecombinant Hepatitis B virus Protein X
See all Protein X proteins and peptides
Purity> 90 % Densitometry.
Endotoxin level < 1.0 EU/µg
Endotoxin level< 1.000 Eu/µg
Expression systemEscherichia coli
Protein lengthFull length protein
SpeciesHepatitis B virus
SequenceMAARVCCQLD PARDVLCLRP VGAESRGRPV SGPFGTLPSP SSSAVPADHG AHLSLRGLPV CAFSSAGPCA LRFTSARRME TTVNAHQVLP KVLHKRTLGL SAMSTTDLEA YFKDCLFKDW EELGEEIRLK VFVLGGCRHK LVCSPAPCNF FTSA
Predicted molecular weight18 kDa
Amino acids1 to 154
TagsHis tag N-Terminus
Our Abpromise guarantee covers the use of ab203540 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
50 mM acetate buffer
ReconstitutionAdd 0.1 M acetate buffer pH 4.0 to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 µg/mL. In higher concentrations the solubility of this antigen is limited. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
- Peptide X
- Protein X
RelevanceProtein X from Hepatitis B virus genotype C subtype adr (strain Japan/adr4/1983) (HBV-C). Multifunctional protein that may modulate protein degradation pathways, apoptosis, transcription, signal transduction, cell cycle progress, and genetic stability by directly or indirectly interacting with hosts factors. Does not seem to be essential for HBV infection. May be directly involved in development of cirrhosis and liver cancer (hepatocellular carcinoma). Most of cytosolic activities involve modulation of cytosolic calcium. The effect on apoptosis is controversial depending on the cell types in which the studies have been conducted. By binding to human DDB1, may affect cell viability and stimulate genome replication. May induce apoptosis by localizing in mitochondria and causing loss of mitochondrial membrane potential. May also modulate apoptosis by binding human CFLAR, a key regulator of the death-inducing signaling complex (DISC). Moderately stimulates transcription of many different viral and cellular transcription elements. Promoters and enhancers stimulated by HBx contain DNA binding sites for NF-kappa-B, AP-1, AP-2, c-EBP, ATF/CREB, or the calcium-activated factor NF-AT. May bind bZIP transcription factors like CREB1
Cellular localizationHost cytoplasm. Host nucleus. Host mitochondrion
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab203540 has been referenced in 3 publications.
- Chen T et al. HBx-related long non-coding RNA 01152 promotes cell proliferation and survival by IL-23 in hepatocellular carcinoma. Biomed Pharmacother 115:108877 (2019). PubMed: 31054511
- Huang Y et al. Hepatitis B Virus X Protein-Induced ROR? Expression to Promote the Migration and Proliferation of Hepatocellular Carcinoma. Biomed Res Int 2019:5407126 (2019). PubMed: 31781621
- Zhang J et al. Serum microRNA-30c levels are correlated with disease progression in Xinjiang Uygur patients with chronic hepatitis B. Braz J Med Biol Res 50:e6050 (2017). PubMed: 28492809