Description

  • Product name

    Recombinant Hepatitis C Virus Genotype 6a NS3 protein
  • Biological activity

    This protein is immunoreactive with sera of HCV-infected individuals.
  • Purity

    > 95 % SDS-PAGE.
    This protein protein was purified by proprietary chromatographic techniques.
  • Expression system

    Escherichia coli
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Amino acids

      1192 to 1459

Specifications

Our Abpromise guarantee covers the use of ab67973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.2% Triton-X-100, 9% Urea, 0.395% Tris HCl, 50% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • HCV genotype 6 NS3
    • NS3P
    • HCV 6
    • HCV6 NS3
    • Serine protease/NTPase/helicase NS3
    see all
  • Relevance

    HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 encompasses amino acid residues approximately 1050 to 1640. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
  • Cellular localization

    Endoplasmic reticulum

References

ab67973 has not yet been referenced specifically in any publications.

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