Recombinant hepatitis c virus Hepatitis C Virus NS3 + NS4A protein (ab198093)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant hepatitis c virus Hepatitis C Virus NS3 + NS4A protein
See all Hepatitis C Virus NS3 + NS4A proteins and peptides -
Biological activity
Assay Conditions: Assay was performed using 100 µl HCV assay buffer containing 50 mM Tris, pH 7.4, 150 mM NaCl, 5 mM DTT, 10% glycerol, and 5 µM fluorescently labeled HCV substrate peptide. Reaction was continuously monitored at room temperature for 20 min. at ex350/em500.
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Purity
>= 80 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Hepatitis C virus -
Sequence
MDYKDDDDKHHHHHHGSVVIVGRIILSGSGSITAYSQQTRGVLGCIITSL TGRDKNQVEGEVQVVSTATQSFLATCINGVCWTVYHGAGSKTLAGPKGPI TQMYTNVDLDLVGWQAPPGARSMTPCSCGSSDLYLVTRHADVIPVRRRGD SRGSLLSPRPVSYLKGSSGGPLLCPSGHVVGVFRAAVCTRGVAKAVDFIP VESMETTMRS -
Predicted molecular weight
22 kDa including tags -
Tags
His-DDDDK tag N-Terminus -
Additional sequence information
Fusion protein corresponding to the serine protease NS3 (a.a. 3-181) and cofactor NS4A (a.a. 21-32) from Hepatitis C virus genotype 1b.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab198093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.79% Tris HCl, 2.9% Sodium chloride, 10% Glycerol (glycerin, glycerine), 0.02% Triton X-100, 0.08% Beta mercaptoethanolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab198093 has not yet been referenced specifically in any publications.