Product nameRecombinant Hepatitis C Virus NS3 + NS4A (mutated D168 V) protein
See all Hepatitis C Virus NS3 + NS4A proteins and peptides
Protein lengthProtein fragment
Amino Acid Sequence
SequenceMDYKDDDDKHHHHHHGSVVIVGRIILSGSGSITAYSQQTRGVLGCIITSL TGRDKNQVEGEVQVVSTATQSFLATCINGVCWTVYHGAGSKTLAGPKGPI TQMYTNVDLDLVGWQAPPGARSMTPCSCGSSDLYLVTRHADVIPVRRRGD SRGSLLSPRPVSYLKGSSGGPLLCPSGHVVGVFRAAVCTRGVAKAVVFIP VESMETTMRS
Molecular weight22 kDa including tags
Modificationsmutated D168 V
TagsHis-DDDDK tag N-Terminus
Additional sequence informationFusion protein of aa21-32 of NS4A, linker (4 aa) and aa3-181 of NS3 with Asp-to-Val mutation on aa168.
- Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (ab1162)
- Anti-DDDDK tag antibody - (Equivalent to FLAG antibodies from Sigma) (ab1257)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-DDDDK tag antibody [F-tag-01] - (Equivalent to FLAG antibodies from Sigma) (ab18230)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-6X His tag® antibody (ab9108)
Our Abpromise guarantee covers the use of ab198098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific Activity: 45 pmole/min/µg.
Assay Conditions: Assay was performed in 100 µl HCV assay buffer containing 50 mM Tris, pH7.4, 150 mM NaCl, 5 mM DTT, 10% glycerol, and 5 µM fluorescently labeled HCV substrate peptide. Reaction was continuously monitored at room temperature for 20 min. at ex350/em500.
Purity> 90 % SDS-PAGE.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 2.32% Sodium chloride, 0.2% Triton-X-100, 0.08% Beta mercaptoethanol, 30% Glycerol
Contains 100 µg/ml DDDDK peptide.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ab198098 has not yet been referenced specifically in any publications.