Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: GST tag N-Terminus
- Suitable for: ELISA, Flow Cyt, SDS-PAGE, WB
Product nameRecombinant Hepatitis C Virus NS3 protein
See all Hepatitis C Virus NS3 proteins and peptides
Biological activityStrongly reacts with human HCV positive serum.
Purity> 95 % SDS-PAGE.
Purity of proteins is evaluated by SDS-PAGE
Expression systemEscherichia coli
Protein lengthProtein fragment
Amino acids1192 to 1456
TagsGST tag N-Terminus
Additional sequence informationHCV polyprotein (genotype 1B)
Our Abpromise guarantee covers the use of ab49022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesStrongly reacts with human HCV positive serum.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 0.2% Triton-X-100, 9% Urea, 0.395% Tris HCl, 50% Glycerol (glycerin, glycerine)
- Hepatitis C Virus NS3
RelevanceHCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
Cellular localizationEndoplasmic reticulum membrane
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab49022 has been referenced in 1 publication.
- Subramaniam AB et al. Metal-amplified Density Assays, (MADAs), including a Density-Linked Immunosorbent Assay (DeLISA). Lab Chip 15:1009-22 (2015). PubMed: 25474561