Description

  • Product name

    Recombinant Hepatitis C Virus NS3 protein
    See all Hepatitis C Virus NS3 proteins and peptides
  • Biological activity

    Strongly reacts with human HCV positive serum.
  • Purity

    > 95 % SDS-PAGE.
    Purity of proteins is evaluated by SDS-PAGE
  • Expression system

    Escherichia coli
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Amino acids

      1192 to 1456

Specifications

Our Abpromise guarantee covers the use of ab49022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Flow Cytometry

    SDS-PAGE

    Western blot

  • Form

    Liquid
  • Additional notes

    Strongly reacts with human HCV positive serum.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.2% Triton-X-100, 9% Urea, 0.395% Tris HCl, 50% Glycerol

General Info

  • Alternative names

    • Hepatitis C Virus NS3
    • NS3
    • NS3P
    • p70
    • Serine protease/NTPase/helicase
    see all
  • Relevance

    HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
  • Cellular localization

    Endoplasmic reticulum membrane

References

ab49022 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry and your interest in our products. Generally, one vial of 100 ug of protein of these products (ab49022, ab49024, ab49025) typically can give 50 to 100 tests depending on the format. More tests can be performed for ELISA and less for lateral flow. However, all depends on the customers’ needs. I hope this helps and if I can assist further, please do not hesitate to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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