Product nameRecombinant Hepatitis C Virus NS3 protein
See all Hepatitis C Virus NS3 proteins and peptides
Protein lengthProtein fragment
Amino Acid Sequence
Amino acids1192 to 1456
Our Abpromise guarantee covers the use of ab49024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityStrongly reacts with human HCV positive serum.
Purity> 95 % SDS-PAGE.
Purity of proteins is evaluated by SDS-PAGE
Additional notesStrongly reacts with human HCV positive serum.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.2% Triton-X-100, 9% Urea, 0.395% Tris HCl, 50% Glycerol
- Hepatitis C Virus NS3
RelevanceHCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
Cellular localizationEndoplasmic reticulum membrane
ab49024 has not yet been referenced specifically in any publications.