• Product name

    Recombinant Hepatitis C Virus NS3 protein (Biotin)
    See all Hepatitis C Virus NS3 proteins and peptides
  • Biological activity

    ab68047 is immunoreactive with sera of HCV-infected individuals.
  • Purity

    > 95 % SDS-PAGE.
    ab68047 was purified by a proprietary chromatographic technique.
  • Expression system

    Escherichia coli
  • Protein length

    Protein fragment
  • Animal free

  • Nature

    • Amino acids

      1450 to 1643
    • Additional sequence information

      The E.coli derived recombinant Biotin Labeled protein contains the HCV NS3 immunodominant regions. The Biotin labeled protein is fused with b-galactosidase (114 kDa) at N-terminus.
  • Conjugation



Our Abpromise guarantee covers the use of ab68047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot


  • Form

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 9% Urea, 0.395% Tris HCl, 0.0292% EDTA, 50% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Hepatitis C Virus NS3
    • NS3
    • NS3P
    • p70
    • Serine protease/NTPase/helicase
    see all
  • Relevance

    HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
  • Cellular localization

    Endoplasmic reticulum membrane


ab68047 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


Thank you for contacting us.

Please accept my apologies for the delay, it was a very comprehensive search.

Unfortunately, I have to let you know that we do not have a suitable HCV NS3 protein available that can be used as a positive control in Western blot with ab40464.

This antibody was generated against genotype 1b-HCV NS3 protein aa1027-1219, the epitope was mapped to aa1186-1219. None of our recombinant HCV NS3 proteins contain this region though.

I am sorry that I cannot be of more assistance on this occasion, but I hope this information is still helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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