Recombinant Hepatitis C Virus NS3 protein (Biotin) (ab68047)
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Description
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Product name
Recombinant Hepatitis C Virus NS3 protein (Biotin)
See all Hepatitis C Virus NS3 proteins and peptides -
Biological activity
ab68047 is immunoreactive with sera of HCV-infected individuals. -
Purity
> 95 % SDS-PAGE.
ab68047 was purified by a proprietary chromatographic technique. -
Expression system
Escherichia coli -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Amino acids
1450 to 1643 -
Additional sequence information
The E.coli derived recombinant Biotin Labeled protein contains the HCV NS3 immunodominant regions. The Biotin labeled protein is fused with b-galactosidase (114 kDa) at N-terminus.
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Conjugation
Biotin
Specifications
Our Abpromise guarantee covers the use of ab68047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
ELISA
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 9% Urea, 0.395% Tris HCl, 0.0292% EDTA, 50% GlycerolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Hepatitis C Virus NS3
- NS3
- NS3P
see all -
Relevance
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa. -
Cellular localization
Endoplasmic reticulum membrane
Datasheets and documents
References
ab68047 has not yet been referenced specifically in any publications.
