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I am performing a direct ELISA for the detection of HCV antigens (core and NS4). SO I would like to know whether Hepatitis C Virus NS4 antibody [1G7] (Biotin) (ab2591) recognizes either Hepatitis C Virus NS4 protein (ab49027) or Hepatitis C Virus NS4 protein (ab67977). Also whether Hepatitis C Virus Core Antigen antibody [1E5] (Biotin) (ab2583) recognizes Hepatitis C Virus Core Antigen protein (ab49015). please attach a standard protocol for direct ELISA for the antigen and antibody that you recommend.
Asked on Jan 30 2009
Thank you for your enquiry. The antibody ab2591 will detect ab49028 and the antibody ab2583 will detect ab49015 in ELISA. A standard protocol for direct ELISA can be found under the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=11388 I hope, this information helps. Please do not hesitate to contact us again, if you have further questions.
Answered on Jan 30 2009