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    recombinant-hepatitis-c-virus-ns4-protein-biotin-ab49030.pdf

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Microbiology Organism Virus RNA Virus ssRNA positive strand virus HCV
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Recombinant Hepatitis C Virus NS4 protein (Biotin) (ab49030)

  • Datasheet
  • SDS
Submit a review Q&A (1)

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Abpromise

Guaranteed product quality, expert customer support.

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE

Description

  • Product name

    Recombinant Hepatitis C Virus NS4 protein (Biotin)
    See all Hepatitis C Virus NS4 proteins and peptides
  • Biological activity

    Reacts strongly with human HCV positive serum
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Amino acids

      1658 to 1863
  • Conjugation

    Biotin

Associated products

  • Related Products

    • Anti-beta Galactosidase antibody (ab616)
    • Biotin Anti-beta Galactosidase antibody (ab6645)
    • Anti-beta Galactosidase antibody (ab9361)

Specifications

Our Abpromise guarantee covers the use of ab49030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Form

    Liquid
  • Additional notes

    Reacts strongly with human HCV positive serum
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 7.2
    Constituents: 48% Urea, 0.078% Beta mercaptoethanol, 0.316% Tris HCl

General Info

  • Alternative names

    • HCV
    • HCV NS4
    • Hepatitis C Virus nonstructural antigen 4
    • Non structural protein 4A
    • Non structural protein 4B
    • NS4A
    • NS4B
    • p27
    • p8
    see all
  • Relevance

    NS4 A/B are two of the seven nonstructural (NS) proteins making up the HCV polyprotein. The NS proteins recruit the viral genome into an RNA replication complex, which is associated with rearranged cytoplasmic membranes. NS4A acts as a cofactor with the NS3 serine protease and stabilizes its folding. The NS3-NS4A complex is essential for the activation of the latter and allows membrane anchorage of NS3. HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. HCV is responsible for a large proportion of worldwide chronic viral hepatitides. Most of these infections develop into chronic hepatitis, which often progresses to liver cirrhosis and hepatocellular carcinoma. At present, (unlike hepatitis A and B), there is no vaccine to prevent hepatitis C infection. The hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is a relatively hydrophobic 27-kDa protein. The 4A protein has a molecular weight of 6 kDa.
  • Cellular localization

    Endoplasmic reticulum; multi-pass membrane protein.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab49030? Please let us know so that we can cite the reference in this datasheet.

ab49030 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

What is the genotype for this protein?

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Abcam community

Verified customer

Asked on Apr 27 2011

Answer

Thank you for your phone call yesterday. I have heard back from my contact at the lab, and the genotype is 1B. I hope this information is helpful. Please let me know if you have any further questions.

Read More

Abcam Scientific Support

Answered on Apr 27 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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