Recombinant Hepatitis C Virus NS4A +B protein (FITC) (ab78143)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: WB, ELISA
Description
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Product name
Recombinant Hepatitis C Virus NS4A +B protein (FITC) -
Biological activity
Reacts strongly with human HCV positive serum. -
Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Amino acids
1658 to 1863
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Specifications
Our Abpromise guarantee covers the use of ab78143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
ELISA
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Form
Liquid -
Additional notes
Reacts strongly with human HCV positive serum. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 48% Urea, 0.078% Beta mercaptoethanol, 0.316% Tris HCl
General Info
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Relevance
Hepatitis C virus (HCV) is a small (55-65 nm in size), enveloped, positive sense single strand RNA virus in the family Flaviviridae. The structure of the hepatitis C virus consists of a core of genetic material (RNA), surrounded by an icosahedral protective shell of protein, and further encased in a lipid (fatty) envelope of cellular origin. Two viral envelope glycoproteins, E1 and E2, are embedded in the lipid envelope. Hepatitis C virus has a positive sense RNA genome that consists of a single open reading frame of 9600 nucleoside bases. At the 5' and 3' ends of the RNA are the UTR regions, that are not translated into proteins but are important to translation and replication of the viral RNA. The 5' UTR has a ribosome binding site (IRES - Internal Ribosomal Entry Site) that starts the translation of a 3000 amino acid containing protein that is later cut by cellular and viral proteases into 10 active structural and non-structural smaller proteins. NS4A is a non-structural viral protein that binds to a portion of HCV protease, inhibits HCV replication.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab78143 has not yet been referenced specifically in any publications.