Recombinant Hepatitis C Virus NS5 protein (ab49032)

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Overview

  • Product name
    Recombinant Hepatitis C Virus NS5 protein
  • Protein length
    Protein fragment

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Amino acids
      2061 to 2302

Specifications

Our Abpromise guarantee covers the use of ab49032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Flow Cytometry

    Western blot

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.2% Triton-X-100, 9% Urea, 0.395% Tris HCl, 50% Glycerol

General Info

  • Alternative names
    • HCV NS5
    • Hepatitis C virus non structural protein 5
    • Hepatitis C virus nonstructural protein 5
    • Hepatitis C Virus NS5
  • Relevance
    Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha. NS5 (non structural protein 5) may play a role in the viral RNA replication of the Hepatitis C Virus.

References

ab49032 has not yet been referenced specifically in any publications.

Publishing research using ab49032? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Do you have by any chance the MW of Hepatitis C Virus NS5 protein (Tagged) (ab49032)

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Answer

Thank you for contacting Abcam regarding ab49032.


I can confirm that along with the tag, this protein is ˜40kDa.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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Question

Dear Abcam Technical support,

Sorry to bother you again but you are a so great source of information.

I would like to develop a AlphaScreen/Lisa using Cyclophilin a that will be tagged with GST and in the other side I need a NS5A peptide ideally tagged with biotin.

I saw that you sell 3 NS5A protein. My questions are:

1. do you think my best option will be ab68096 that is described in your web as full length conjugated with biotin?

2. the product ab49032 is described as aa2061 to 2302 with an N-terminal tag. What is the tag?

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Answer

Thank you for contacting Abcam

Please do not think you are bothering us, it’s what we are here for.

In regard to your questions, I would agree with you that ab68096 is a really good option for your ELISA and the tag of ab79032 is a GST tag.

If there is anything else we can help you with, please let me know.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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