Recombinant Hepatitis C Virus NS5 protein (ab49032)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Suitable for: ELISA, Flow Cyt, WB

Description

  • Product name

    Recombinant Hepatitis C Virus NS5 protein

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Amino acids

      2061 to 2302

Specifications

Our Abpromise guarantee covers the use of ab49032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Flow Cytometry

    Western blot

  • Form

    Liquid

  • Additional notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.

    pH: 7.2
    Constituents: 0.2% Triton-X-100, 9% Urea, 0.395% Tris HCl, 50% Glycerol (glycerin, glycerine)

General Info

  • Alternative names

    • HCV NS5
    • Hepatitis C virus non structural protein 5
    • Hepatitis C virus nonstructural protein 5
    • Hepatitis C Virus NS5

  • Relevance

    Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha. NS5 (non structural protein 5) may play a role in the viral RNA replication of the Hepatitis C Virus.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab49032? Please let us know so that we can cite the reference in this datasheet.

ab49032 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Do you have by any chance the MW of Hepatitis C Virus NS5 protein (Tagged) (ab49032)

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Answer

Thank you for contacting Abcam regarding ab49032.


I can confirm that along with the tag, this protein is ˜40kDa.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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Question

Dear Abcam Technical support,

Sorry to bother you again but you are a so great source of information.

I would like to develop a AlphaScreen/Lisa using Cyclophilin a that will be tagged with GST and in the other side I need a NS5A peptide ideally tagged with biotin.

I saw that you sell 3 NS5A protein. My questions are:

1. do you think my best option will be ab68096 that is described in your web as full length conjugated with biotin?

2. the product ab49032 is described as aa2061 to 2302 with an N-terminal tag. What is the tag?

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Answer

Thank you for contacting Abcam

Please do not think you are bothering us, it’s what we are here for.

In regard to your questions, I would agree with you that ab68096 is a really good option for your ELISA and the tag of ab79032 is a GST tag.

If there is anything else we can help you with, please let me know.

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