Key features and details
- Expression system: Escherichia coli
- Purity: > 97% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, HPLC, SDS-PAGE
Product nameRecombinant herpes simplex virus viral MIP2 protein
See all viral MIP2 proteins and peptides
Fully biologically active when compared to standard.
Determined by the inhibitory effect on monocyte migration response to Human MIP-1 alpha using a concentration range of 1.0µg-10.0µg/ml of viral MIP2 will inhibit 25ng/ml of Human MIP-1 alpha.
Purity> 97 % SDS-PAGE.
> 97 % by HPLC.
Endotoxin level< 1.000 Eu/µg
Expression systemEscherichia coli
Protein lengthFull length protein
SpeciesHerpes simplex virus
SequenceLGASWHRPDK CCLGYQKRPL PQVLLSSWYP TSQLCSKPGV IFLTKRGRQV CADKSKDWVK KLMQQLPVTA
Predicted molecular weight8 kDa
Amino acids24 to 93
Additional sequence informationHuman herpesvirus 8. This product is for the mature full length protein. The signal peptide is not included.
Our Abpromise guarantee covers the use of ab201382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C long term. Avoid freeze / thaw cycle.
Constituents: 0.87% Sodium chloride, 99% Phosphate Buffer
Briefly centrifuge prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionBriefly centrifuge prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
- Viral macrophage inflammatory protein II
- vMIP 1B
- vMIP II
RelevancevMIP2 is a chemokine analog encoded by the human herpesvirus (HHV8) and is a potent in vitro antagonist of many chemokine receptors. In vivo vMIP2 has been shown to be a potent inhibitor of type 1 T-cell-mediated inflammation. Three chemokine-like proteins, vMIP-I, vMIP-II and vMIP-III are encoded within the HHV8 genome. Among human chemokines, vMIP2 is most closely related to MIP-1a, sharing approximately 41% amino acid sequence identity. The CC chemokine receptor (CCR) 8 belongs to the seven transmembrane-spanning receptor families and functionally responds to the eukaryotic CC chemokines I-309, MIP 1b and vMIPI and vMIP2. Both vMIP I and vMIP2 partially block HIV infection of peripheral blood mononuclear cells. vMIPI and vMIP2 are also highly angiogenic. Chemokines play a profound role in leukocyte trafficking and the development of adaptive immune responses. Perhaps due to their importance in host defense, viruses have adopted many of the hallmarks displayed by chemokines. One therapeutic strategy to prevent accumulation of pro-inflammatory immune cells is the use of specific chemokine receptor antagonists. An interesting and promising candidate in this context is the viral antagonist vMIP2 as this molecule acts on a broad spectrum of chemokine receptors.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab201382 has not yet been referenced specifically in any publications.