Description

  • Product name

    Recombinant Hirudin protein (Active)
    See all Hirudin proteins and peptides
  • Biological activity

    Hirudin is fully biologically active when compared to standard. Its specific activity is >1×104ATU/mg.

  • Purity

    > 96 % SDS-PAGE.
    >96% HPLC analyses.
  • Endotoxin level

    < 0.010 Eu/µg
  • Expression system

    Saccharomyces cerevisiae
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Poecilobdella viridis
    • Predicted molecular weight

      7 kDa
    • Amino acids

      1 to 65
    • Additional sequence information

      Recombinant Hirundin variant, identical to natural Hirundin, but for substitution of Leu to Ile at N terminal end and absence of sulphate group on Tyr63.

Specifications

Our Abpromise guarantee covers the use of ab201396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    HPLC

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

    Upon reconstitution, the preparation is stable for up to one week at 2-8°C.

    For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.00
    Constituents: 2% Mannitol, PBS

    Lyophilized from a 0.2 µm filtered concentrated solution

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20C. Further dilutions should be made in appropriate buffered solutions.

General Info

  • Alternative names

    • Hirudin 1
    • Lepirudin
    • Refludan
  • Relevance

    Hirudin is a highly effective and very specific anticoagulant which specifically inhibits formation of fibrin and activation of factors V, VIII and XIII by forming irreversible, noncovalent 1:1 complexes with a-thrombin. Hirudin is a 65 amino acid polypeptide, 7 kDa protein naturally originating from the medical leech Hirudo medicinalis. It is the most potent thrombin inhibitor known to date.
  • Cellular localization

    Secreted

References

ab201396 has not yet been referenced specifically in any publications.

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