Recombinant HIV-1 gp120 (Du172.17 (mutated E46G, T396A + A497T) protein (ab167715)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: ELISA, SDS-PAGE
Description
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Product name
Recombinant HIV-1 gp120 (Du172.17 (mutated E46G, T396A + A497T) protein -
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized ab167715 at 5 μg/mL (100 μL/well) can bind Recombinant human CD4 protein (Active) (ab167756) with a linear range of 1-39 ng/mL.
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human immunodeficiency virus -
Sequence
The predicted N-terminus is Thr36. -
Predicted molecular weight
54 kDa including tags -
Amino acids
36 to 507 -
Modifications
mutated E46G + T396A + A497T -
Tags
His tag C-Terminus -
Additional sequence information
Recombinant Human immunodeficiency virus HIV-1 gp120 protein derived from the envelope gene of HIV-1 strain CN54 gp160 and glycosylated with N-linked sugars and expressed in HEK293 cells.
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Description
Recombinant human immunodeficiency virus HIV-1 gp120 (Du172.17 (mutated E46G + T396A + A497T) protein (Active)
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab167715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
SDS-PAGE
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Form
Lyophilized -
Additional notes
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
pH: 7.40
Constituent: 100% PBS
Normally Mannitol or Trehalose are added as protectants before lyophilizationThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
General Info
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Alternative names
- gp120
- gp120 glycoprotein
- Human Immunodeficiency Virus 1
see all -
Relevance
HIV1 is equipped with the envelope gp160 glycoprotein for interaction with Langerhans cells (LCs) and dendritic cells (DCs), the members of the innate immune system, which confront the virus at the portal of virus entry in the human body. These cells are equipped with receptors by which they bind and endocytose the virus. The gp120 glycoprotein is used for binding to CD4 receptor and CCR5 co-receptor of T helper 2 (Th2) cells, and is able to induce FcepsilonRI(+) hematopoietic cells to produce IL4, which inactivates the host adaptive immune response. -
Cellular localization
Cell Membrane
Images
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SDS-PAGE of reduced ab167715 stained overnight with Coomassie Blue.
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Immobilized ab167715 at 5 μg/mL (100 μL/well) can bind Recombinant human CD4 protein (Active) (ab167756) with a linear range of 1-39 ng/mL.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab167715 has been referenced in 1 publication.
- Dodagatta-Marri E et al. Protein-Protein Interaction between Surfactant Protein D and DC-SIGNviaC-Type Lectin Domain Can Suppress HIV-1 Transfer. Front Immunol 8:834 (2017). PubMed: 28824609