Recombinant HIV tat (mutated C22) protein (ab83352)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: Blocking, Functional Studies, WB, SDS-PAGE
Description
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Product name
Recombinant HIV tat (mutated C22) protein
See all HIV tat proteins and peptides -
Biological activity
Reacts with anti-Tat antibodies from human, monkey, rabbit and mouse serum. -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Modifications
mutated C22
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab83352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Blocking
Functional Studies
Western blot
SDS-PAGE
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Form
Lyophilized -
Additional notes
Reacts with anti-Tat antibodies from human, monkey, rabbit and mouse serum. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C.
pH: 7.50
Constituents: 0.24% Monobasic dihydrogen sodium phosphate, 0.91% Mannitol, 0.284% Dibasic monohydrogen sodium phosphate, 0.328% Sodium phosphate, 0.0154% DTT, 2.5% Glycerol, 6.96% Sodium chloride -
ReconstitutionThe protein should be reconstituted in apirogenic and sterile dH2O. The reconstituted solution has to be used immediately, since it is not stable in liquid form.
General Info
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Alternative names
- p14
- Tat
- Tat protein
- Transactivating regulatory protein
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Relevance
The transcriptional transactivator (Tat) is a key regulatory protein of HIV. It is expressed early after the virus integrates into the cell, and stimulates the elongation of RNA polymerase II. It binds onto a sequence known as the TAR, or transactivator response element, located at the end of the HIV genetic chain. There, the tat protein helps assemble new copies of HIV. The tat protein-TAR complex speeds up the rate of viral reproduction by about a thousand times. If it is not present, the transcription process frequently stops short, and few functional HIV particles are produced. Tat is an important potential target for antiretrovirals and vaccine development.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab83352 has been referenced in 1 publication.
- El-Amine R et al. HIV-1 Tat protein induces DNA damage in human peripheral blood B-lymphocytes via mitochondrial ROS production. Redox Biol 15:97-108 (2018). PubMed: 29220699