Description

  • Product name

    Recombinant HIV tat (mutated C22) protein
    See all HIV tat proteins and peptides
  • Biological activity

    Reacts with anti-Tat antibodies from human, monkey, rabbit and mouse serum.
  • Purity

    > 90 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Modifications

      mutated C22

Specifications

Our Abpromise guarantee covers the use of ab83352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking

    Functional Studies

    Western blot

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

    Reacts with anti-Tat antibodies from human, monkey, rabbit and mouse serum.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C.

    pH: 7.50
    Constituents: 0.24% Monobasic dihydrogen sodium phosphate, 0.91% Mannitol, 0.284% Dibasic monohydrogen sodium phosphate, 0.328% Sodium phosphate, 0.0154% DTT, 2.5% Glycerol, 6.96% Sodium chloride

  • Reconstitution
    The protein should be reconstituted in apirogenic and sterile dH2O. The reconstituted solution has to be used immediately, since it is not stable in liquid form.

General Info

  • Alternative names

    • p14
    • Tat
    • Tat protein
    • Transactivating regulatory protein
  • Relevance

    The transcriptional transactivator (Tat) is a key regulatory protein of HIV. It is expressed early after the virus integrates into the cell, and stimulates the elongation of RNA polymerase II. It binds onto a sequence known as the TAR, or transactivator response element, located at the end of the HIV genetic chain. There, the tat protein helps assemble new copies of HIV. The tat protein-TAR complex speeds up the rate of viral reproduction by about a thousand times. If it is not present, the transcription process frequently stops short, and few functional HIV particles are produced. Tat is an important potential target for antiretrovirals and vaccine development.

References

This product has been referenced in:

  • El-Amine R  et al. HIV-1 Tat protein induces DNA damage in human peripheral blood B-lymphocytes via mitochondrial ROS production. Redox Biol 15:97-108 (2018). Read more (PubMed: 29220699) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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