Recombinant HIV tat (mutated C31A + C34A) protein (ab119447)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: WB, SDS-PAGE
Description
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Product name
Recombinant HIV tat (mutated C31A + C34A) protein
See all HIV tat proteins and peptides -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Modifications
mutated C31A + C34A -
Additional sequence information
Recombinant HIV-1 TAT Clade B It Is a single, non-glycosylated, polypeptidic chain containing 86 amino acids encoded by two exons.
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Specifications
Our Abpromise guarantee covers the use of ab119447 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C.
Constituent: 0.1% Glycerol
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Reconstitutionab119447 should be reconstituted in apirogenic sterile water or PBS buffer. Reconstituted protein should be used immediately.
General Info
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Alternative names
- p14
- Tat
- Tat protein
- Transactivating regulatory protein
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Relevance
The transcriptional transactivator (Tat) is a key regulatory protein of HIV. It is expressed early after the virus integrates into the cell, and stimulates the elongation of RNA polymerase II. It binds onto a sequence known as the TAR, or transactivator response element, located at the end of the HIV genetic chain. There, the tat protein helps assemble new copies of HIV. The tat protein-TAR complex speeds up the rate of viral reproduction by about a thousand times. If it is not present, the transcription process frequently stops short, and few functional HIV particles are produced. Tat is an important potential target for antiretrovirals and vaccine development.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab119447 has not yet been referenced specifically in any publications.