Recombinant HIV1 gp41 protein (ab49070)

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ELISA - Recombinant HIV1 gp41 protein (ab49070)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Suitable for: ELISA, WB

    Description

    • Product name

      Recombinant HIV1 gp41 protein
      See all HIV1 gp41 proteins and peptides

    • Purity

      > 90 % SDS-PAGE.
      Purified by affinity chromatography.

    • Expression system

      Escherichia coli

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Sequence

        IEFPGIFRPG GGDMRDNWRS ELYKYKVVKI EPLGVAPTKA KRRVVQREKR AVGIGALFLG FLGAAGSTMG AASMTLTVQA RQLLSGIVQQ QNNLLRAIEA QQHLLQLTVW GIKQLQARIL AVERYLKDQQ LLGIWGCSGK LICTTAVPWN ASWSNKSLEQ IWNNMTWMEW DREINNYTSL IHSLIEESQN QQEKNEQEL LELDKWASLW NWFNITNWLW YIKLFIMIVG GLVGLRIVFA VLSVVNRVRQ GYSPLSFQTH LPIPRGPDRP EGIEEEGGER DRDRSIRLVN GSLALIWDDL RSLCLFSYHR LRDLLLIVTR IVELLGRRGW EALKYWWNLL QYWSQELKNS AVSLLNATAI AVAEGTDRVI EVVQGAYRAI RHIPRRIRQG LERILL

      • Additional sequence information

        Tag: Beta galactosidase N-terminus.

    Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab49070 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      Western blot

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

      pH: 7.2
      Preservative: 0.01% Sodium azide
      Constituents: 9% Urea, 0.395% Tris HCl, 0.0292% EDTA, 50% Glycerol (glycerin, glycerine)

    General Info

    • Alternative names

      • Env
      • Glycoprotein 41
      • gp41
      • Human Immunodeficiency Virus 1
      • TM
      • Transmembrane protein
      see all

    • Relevance

      gp41/120 is the major HIV protein associated with the HIV envelope. It functions as the viral antireceptor or attachment protein. gp41 (or TM) traverses the envelope, whereas gp120 is present on the outer surface and is noncovalently attached to gp41. The precursor of gp120/41 (gp160) is synthesized in the endoplasmic reticulum and is transported via the golgi body to the cell surface. Upon activation of the envelope glycoprotein (gp120/41) by cellular receptors, gp41 undergoes conformational changes that mediate fusion of the viral and cellular membranes.

    Images

    • ELISA - Recombinant HIV1 gp41 protein (ab49070)
      ELISA - Recombinant HIV1 gp41 protein (ab49070)

      ELISA using ab49070 at 2µg/mL Antibodies which recognise the 2F5 and 4E10 epitope were used as primary antibodies, and Protein A HRP was used as a seconday antibody.

      Significance: gp41-specific antibodies which bind two different epitopes are able to recognize this full length gp41 in ELISA

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab49070? Please let us know so that we can cite the reference in this datasheet.

    ab49070 has been referenced in 1 publication.

    • Caires AJ  et al. Gold nanoparticle-carboxymethyl cellulose nanocolloids for detection of human immunodeficiency virus type-1 (HIV-1) using laser light scattering immunoassay. Colloids Surf B Biointerfaces 177:377-388 (2019). PubMed: 30785035

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    where is tag located?

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    Answer

    Thank you for contacting us. The tag is C terminal.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    Quick question,   How large are each of these proteins?  I would like to know where the dominant band will be on a gel.   Thanks!  

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    Answer

    Thank you for your inquiry. I heard back from the lab regarding ab49070. The total MW of this antigen is around 130 kDa, 30 kDa is the HIV gp41 region and the rest is inactive beta-galactosidase tag. I hope this information helps. I'm still waiting for word on ab43024 and will be in touch again once I hear back.

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