Recombinant HIV1 gp41 protein (ab49070)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: ELISA, WB
Description
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Product name
Recombinant HIV1 gp41 protein
See all HIV1 gp41 proteins and peptides -
Purity
> 90 % SDS-PAGE.
Purified by affinity chromatography. -
Expression system
Escherichia coli -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Sequence
IEFPGIFRPG GGDMRDNWRS ELYKYKVVKI EPLGVAPTKA KRRVVQREKR AVGIGALFLG FLGAAGSTMG AASMTLTVQA RQLLSGIVQQ QNNLLRAIEA QQHLLQLTVW GIKQLQARIL AVERYLKDQQ LLGIWGCSGK LICTTAVPWN ASWSNKSLEQ IWNNMTWMEW DREINNYTSL IHSLIEESQN QQEKNEQEL LELDKWASLW NWFNITNWLW YIKLFIMIVG GLVGLRIVFA VLSVVNRVRQ GYSPLSFQTH LPIPRGPDRP EGIEEEGGER DRDRSIRLVN GSLALIWDDL RSLCLFSYHR LRDLLLIVTR IVELLGRRGW EALKYWWNLL QYWSQELKNS AVSLLNATAI AVAEGTDRVI EVVQGAYRAI RHIPRRIRQG LERILL -
Additional sequence information
Tag: Beta galactosidase N-terminus.
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Specifications
Our Abpromise guarantee covers the use of ab49070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
Western blot
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% Urea, 0.395% Tris HCl, 0.0292% EDTA, 50% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- Env
- Glycoprotein 41
- gp41
see all -
Relevance
gp41/120 is the major HIV protein associated with the HIV envelope. It functions as the viral antireceptor or attachment protein. gp41 (or TM) traverses the envelope, whereas gp120 is present on the outer surface and is noncovalently attached to gp41. The precursor of gp120/41 (gp160) is synthesized in the endoplasmic reticulum and is transported via the golgi body to the cell surface. Upon activation of the envelope glycoprotein (gp120/41) by cellular receptors, gp41 undergoes conformational changes that mediate fusion of the viral and cellular membranes.
Images
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ELISA - Recombinant HIV1 gp41 protein (ab49070)
ELISA using ab49070 at 2µg/mL Antibodies which recognise the 2F5 and 4E10 epitope were used as primary antibodies, and Protein A HRP was used as a seconday antibody.
Significance: gp41-specific antibodies which bind two different epitopes are able to recognize this full length gp41 in ELISA
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab49070 has been referenced in 2 publications.
- Caires AJ et al. Gold nanoparticle-carboxymethyl cellulose nanocolloids for detection of human immunodeficiency virus type-1 (HIV-1) using laser light scattering immunoassay. Colloids Surf B Biointerfaces 177:377-388 (2019). PubMed: 30785035
- Miles P et al. In vitro assembly of a viral envelope. Soft Matter 11:7722-7 (2015). PubMed: 26299568
