Description

  • Product name

    Recombinant HIV1 p24 protein
    See all HIV1 p24 proteins and peptides
  • Purity

    > 90 % SDS-PAGE.
    ab127888 is highly purified by several steps of chromatography.
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Sequence

      PIVQNLQG QMVHQAISPR TLNAWVKVVE EKAFSPEVIP MFSALSEGAT PQDLNTMLNT VGGHQAAMQM LKETINEEAA EWDRLHPVHA GPIAPGQMRE PRGSDIAGTT STLQEQIGWM THNPPIPVGE IYKRWIILGL NKIVRMYSPT SILDIRQGPK EPFRDYVDRF YKTLRAEQAS QEVKNWMTET LLVQNANPDC KTILKALGPG ATLEEMMTAC QGVGGPGHKA RVL
    • Predicted molecular weight

      24 kDa
    • Amino acids

      133 to 363

Specifications

Our Abpromise guarantee covers the use of ab127888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    Additional Application Information

    1. In Western Blotting as a standard for p24
    2. In ELISA assay as a standard in titration of p24 antigen
    3. It can be used in the studies of structure and function of HIV-1 virus as it constitutes HIV-1 core as a capsid protein since it is soluble under physiological conditions.
    4. In SDS-Page as a p24 marker

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20ºC.

    pH: 7.50
    Constituents: 0.078% Beta mercaptoethanol, 0.32% Tris HCl, 50% Glycerol, 0.29% Sodium chloride

General Info

  • Alternative names

    • CA
    • Capsid protein p24
    • HIV-1 Gag p24
    • HIV1gp1
    • Human immunodeficiency virus 1
    • Human immunodeficiency virus type 1 p24
    see all
  • Relevance

    HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • Cellular localization

    Membrane

Images

  • SDS-PAGE analysis of ab127888.

References

ab127888 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.
I have checked some of the HIV1 p24 proteins available in our catalogue with my lab colleagues. Regarding their suitability for ELISPOT, I am afraid it hasn’t been tested in our lab, but due to their suitability in ELISA, they should also work in ELISPOT.
As an example, ab127888 is highly specific in ELISA, but ab73768 or ab43037 are also very likely to work in this assay.
If you would be interested, you could participate in our Antibody evaluation program, by which you test an antibody in a new species/application (such as any of the referred antibodies in ELISPOT), and share the results with us (submitting an Abreview), and you beneficiate from a free antibody.
If you want to read more about the Abreview system, please have a look at the next link:
https://www.abcam.com/abreviews
Please let me know if you would be interested in this program, and if you have any other doubts about it. I am happy to help, and clarify it further if necessary.
Have a very nice day!

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Answer

Thank you for your inquiry.

The p24 protein was expressed as Gag-Pol polyprotein in E. coli and p24 was processed from the polyprotein in E.coli by HIV-1protease coexpressed in the same E. coli cell. There is no tag nor any additional amino acids attached. The product has the same amino acid sequence as the native p24 produced in HIV-1 infected cells. The origin of the clone is pNL4-3, which can produce infective virus of subtype B, upon transfection. The exact amino acid sequence in the polyprotein can be found in

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GenBank: http://www.ncbi.nlm.nih.gov/protein/328419?log$=activityhttp://www.ncbi.nlm.nih.gov/protein/328419?log$=activity

This protein was described and used in these references:

Tanaka N et al ”A simple method for overproduction and purification of p24 Gag protein of human immunodeficiency virus type 1.” Microbiol Immunol 36: 823-831 (1992) PMID:http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=DetailsSearch&term=Microbiol.+Immunol.+36,823-831,1992&log$=activity
Saito A et al “Overproduction, purification, and diagnostic use of the recombinant HIV-1 Gag proteins, the precursor protein p55 and the processed products p17, p24, and p15.” Microbiol Immunol 39:473-483 (1995) PMID: http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=DetailsSearch&term=Microbiol.+Immunol.+39,473-483,1995&log$=activity



I hope this information helps. Please contact us with any other questions.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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