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    recombinant-hiv1-p24-protein-ab43037.pdf

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Microbiology Organism Virus RNA Virus ssRNA positive strand virus HIV
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Recombinant HIV1 p24 protein (ab43037)

  • Datasheet
  • SDS
Submit a review Q&A (10)References (3)

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SDS-PAGE - Recombinant HIV1 p24 protein (ab43037)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Suitable for: ELISA, WB

    You may also be interested in

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    Description

    • Product name

      Recombinant HIV1 p24 protein
      See all HIV1 p24 proteins and peptides
    • Biological activity

      Immunoreactive with all sera of HIV1 infected individuals.
    • Purity

      > 95 % SDS-PAGE.
      Organic extraction > S-Sepharose > S-300.
    • Expression system

      Escherichia coli
    • Accession

      P05888
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Sequence

        PIVQN IQGQMVHQAI SPRTLNAWVK VVEEKAFSPE VIPMFSALSE GATPQDLNTM LNTVGGHQAA MQMLKETINE EAAEWDRLHP AHAGPIAPGQ MREPRGSDIA GTTSTLQEQI GWMTNNPPIP VGEIYKRWII LGLNKIVRMY SPSSILDIRQ GPKEPFRDYV DRFYKTLRAE QASQEVKNWM TETLLVQNAN PDCKTILKAL GPAATLEEMM TACQGVGGPG HKARVL
      • Predicted molecular weight

        26 kDa

    Associated products

    • Related Products

      • Anti-HIV1 p24 antibody (ab19961)
      • HRP Anti-HIV1 p24 antibody (ab20365)
      • FITC Anti-HIV1 p24 antibody (ab20569)
      • Biotin Anti-HIV1 p24 antibody (ab20774)

    Specifications

    Our Abpromise guarantee covers the use of ab43037 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      Western blot

    • Form

      Liquid
    • Additional notes

      Immunoreactive with all sera of HIV-1 infected individuals.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

      pH: 8.5
      Constituents: 48% Urea, 0.605% Tris

    General Info

    • Alternative names

      • CA
      • Capsid protein p24
      • HIV-1 Gag p24
      • HIV1gp1
      • Human immunodeficiency virus 1
      • Human immunodeficiency virus type 1 p24
      see all
    • Relevance

      HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
    • Cellular localization

      Membrane

    Images

    • SDS-PAGE - Recombinant HIV1 p24 protein (ab43037)
      SDS-PAGE - Recombinant HIV1 p24 protein (ab43037)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab43037? Please let us know so that we can cite the reference in this datasheet.

    ab43037 has been referenced in 3 publications.

    • Rosemary Bastian A  et al. Mechanism of multivalent nanoparticle encounter with HIV-1 for potency enhancement of peptide triazole virus inactivation. J Biol Chem 290:529-43 (2015). PubMed: 25371202
    • Gesner M  et al. Fluorescence-linked Antigen Quantification (FLAQ) Assay for Fast Quantification of HIV-1 p24Gag. Bio Protoc 4:N/A (2014). PubMed: 27525295
    • Hughes AJ & Herr AE Microfluidic Western blotting. Proc Natl Acad Sci U S A : (2012). SDS-PAGE . PubMed: 23223527

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 10 Abreviews or Q&A

    Question

    What is the accession number of this protein and what is the amino acid range? What strain is it derived from?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 22 2014

    Answer

    Unfortunately we are unable to share the specific sequence but it is available in the literature. The protein is derived from group MN. These regions are highly conserved throughout all strains.

    Read More

    Kevin Hanson

    Abcam Scientific Support

    Answered on Feb 22 2014

    Question

    Has the activity of this protein been tested?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 09 2013

    Answer

    Thank you for contacting us.


    The immune-biological activity of HIV p24 is derived from its linear AA sequences and has no secondary structure related activity. This protein has not been tested for functional activity.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    -----------------------------------------------------------------------------------------------

    Do you wish running an ELISA was fast and easy? Check out our new SimpleStep™ ELISA, a single wash, colorimetric sandwich ELISA assay.

    Discover more at www.abcam.com/simplestep

    Read More

    Heather Allen

    Abcam Scientific Support

    Answered on Dec 09 2013

    Question

    The product is in 8M urea for the stock solution. My concern was, when the antigen is diluted to pg/mL level with a buffer without urea, is this antigen still stable or remain in the conformation that can be detected by ELISA?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 28 2013

    Answer

    You are correct, ab43037 is an antigen, not an antibody, and it is suitable to be coated on an ELISA plate from 100 ng to 10 ug. The dilution buffer that you use does not have to contain urea, as long as you are coating the wells within the range given above.

    Read More

    Kevin Hanson

    Abcam Scientific Support

    Answered on Jun 28 2013

    Question

    If this antibody is diluted further so the Urea concentration is low enough to not interfere with an ELISA, would the protein still be stable? I am concerned about the high Urea concentration interfering with the ELISA, so I want to make sure I dilute it sufficiently.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 27 2013

    Answer


    ab43037 works well in ELISA at nano to tens of micro gram levels. Thus, dilutions within that range should work sufficiently.

    Read More

    Kevin Hanson

    Abcam Scientific Support

    Answered on Jun 27 2013

    Question

    what concentration for ELISA?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 13 2013

    Answer

    Thank you for contacting us.

    The laboratory provided the following ranges for ELISA. Using ab43037 protein use 100 ng to 10 ug to coat the plate, detection with ab81433 use at 1/5000-1/10000.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Feb 13 2013

    Question

    Hi,
    I am interested in buy a p24 HIV recombinant protein to be used in a ELISPOT assay. Could you please tell me if you have any product that you known it is working in that assay and without inspecific response? We already buy one product from another company and we have a in specific response.

    Thank you in advance for the help

    Read More

    Abcam community

    Verified customer

    Asked on Nov 02 2012

    Answer

    Thank you for contacting us.
    I have checked some of the HIV1 p24 proteins available in our catalogue with my lab colleagues. Regarding their suitability for ELISPOT, I am afraid it hasn’t been tested in our lab, but due to their suitability in ELISA, they should also work in ELISPOT.
    As an example, ab127888 is highly specific in ELISA, but ab73768 or ab43037 are also very likely to work in this assay.
    If you would be interested, you could participate in our Antibody evaluation program, by which you test an antibody in a new species/application (such as any of the referred antibodies in ELISPOT), and share the results with us (submitting an Abreview), and you beneficiate from a free antibody.
    If you want to read more about the Abreview system, please have a look at the next link:
    https://www.abcam.com/abreviews
    Please let me know if you would be interested in this program, and if you have any other doubts about it. I am happy to help, and clarify it further if necessary.
    Have a very nice day!

    Read More

    Abcam Scientific Support

    Answered on Nov 02 2012

    Question

    Do you have any ELISA kit for detection HIV-1 P24 antigen human IgG ?
    Sample : Human serum
    Thank you.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 08 2012

    Answer

    Thank you for contacting us.

    Unfortunately, we do not have a kit for this purpose, but we have two products which may interest you:

    https://www.abcam.com/HIV1-p24-antibody-3954A-ab9071.html (or use the following: https://www.abcam.com/HIV1-p24-antibody-3954A-ab9071.html). This antibody has a good customer review and was used in some publications.

    As a positive control I would like to suggest

    https://www.abcam.com/Recombinant-HIV1-p24-protein-ab43037.html (or use the following: https://www.abcam.com/Recombinant-HIV1-p24-protein-ab43037.html).

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Aug 08 2012

    Question

    Thank you for your reply. it is very helpful.   The research I will carry out is to apply the magnetic detection echnique into HIV detection through sandwich assay.   From the information you provide, HIV1 p24 protein (ab73768) is the antigen I need, right? Since it can be detected by all three antibodies  Anti-HIV1 p24 antibody [ND1] (ab8246) and  Anti-HIV1 p24 antibody [ND1] (FITC) (ab81433) and Anti-HIV1 p24 antibody [39/5.4A] (ab9071).   I wonder if the ab81433 is the same as ab8246 except the ab81433 has FITC fluorogen which could be detected by fluoresence absorption. Right?      Looking for your reply. Thank you!    

    Read More

    Abcam community

    Verified customer

    Asked on Nov 18 2011

    Answer

    Thank you for your reply. Unfortunately, I would not recommend the protein ab73768. This is only a fragment of the HIV1 p24 that lacks large parts of the protein such as the N-terminal amino acids (1-76). If the antibodies you would like to test recognise an epitope within these missing parts (which we do not know as they have not been mapped), these antibodies would not be suitable for the proposed assay. Therefore, I would recommend to use the protein ab43037 which is the full-length HIV1 p24 and will be detected by the antibodies. Indeed, I can confirm that ab8246 and ab81433 are the same antibody clone, thus detecting the same epitope, and that ab81433 is conjugated to FITC which can be used in applications such as immunocytochemistry/immunofluorescence (ICC/IF). For your experiments it seems that you do not need the fluorochrome, so I would recommend ab8246. In summary, for the sELISA two antibodies that recognise different epitopes are required and the matching pair needs to be determined by the end user. To our knowledge, neither ab43037 nor ab8246/ab81433 nor ab9071 have yet been tested in sELISA. Therefore, I can offer a discount off a future purchase if you buy one or all of the products now, test it/them in sELISA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and which product you would like to test sELISA. I will then send a discount code. This code must be issued before purchasing the product(s) so please wait for my reply before ordering. 2. Purchase the product(s) either by phone, fax, or online (www.abcam.com). 3. Test it/them in sELISA. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody and protein ordered, respectively, and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the product(s) turn out to be unsuitable for sELISA, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Nov 18 2011

    Question

    What is the difference between this four products? ab43037 (HIV1 p24 protein) ab73028 HIV1 p24 protein (His tag) ab43036 HTLV I p24 protein ab73768 HIV1 p24 protein I wantt to buy one HIV antigen, and two HIV monoclonal antiboies for magnetic sandwich assay. Could you suggest one kit for me? (not elisa kit)

    Read More

    Abcam community

    Verified customer

    Asked on Nov 17 2011

    Answer

    Thank you for contacting us. The differences are as follows: ab43036 HTLV I p24 protein: This protein is the full length sequence of HTLV I p24, i.e. an in E. coli derived recombinant protein. The Human T-lymphotropic virus Type I (HTLV-1) is not identical with HIV. When Human immunodeficiency virus (HIV), the virus that causes AIDS, was characterized, it was originally named HTLV-III. Therefore, this protein seems not suitable for your experiments. ab43037: This protein is the recombinant full length protein HIV1 p24, expressed in E.coli. This protein is guaranteed to work in Western blot and ELISA. ab73028: This protein is the recombinant full length HIV1 p24 from HxB2 strain purified from Pichia pastoris. It contains a 6x histidine fusion partner which for example facilitates purification. ab73768: This protein is only a recombinant fragment (amino acids 77-436) of the HIV1 p24 protein and is additionally fused to beta-galactosidase. If you could provide some more information on the type of assay you would like to perform and let us know whether you are interested in HIV-1 or HIV-2, I would be happy to make a specific recommendation. In case the assay is similar to a sandwich ELISA (sELISA), I can let you know that we have tested neither the proteins nor any antibodies for their suitability in sELISA, as this has to be tested for each pair individually. In this case, I would suggest to test ab43037 with the antibody clone [ND1] as in ab8246 or ab81433 and for example the monoclonal antibody ab9071. https://www.abcam.com/HIV1-p24-antibody-ND1-ab8246.html https://www.abcam.com/HIV1-p24-antibody-ND1-ab8246.html. https://www.abcam.com/HIV1-p24-antibody-ND1-FITC-ab81433.html https://www.abcam.com/HIV1-p24-antibody-ND1-FITC-ab81433.html. https://www.abcam.com/HIV1-p24-antibody-3954A-ab9071.html https://www.abcam.com/HIV1-p24-antibody-3954A-ab9071.html. If you decide to go ahead and purchase one of these products, please contact our Scientific Support team prior to the purchase as you might be eligible for our testing discount program. Do not hesitate to contact us with more information to select the most suitable products for this experiment. I look forward to your reply.

    Read More

    Abcam Scientific Support

    Answered on Nov 17 2011

    Question

    I'm doing an ELISPOT-type assay with these products and am looking for a protocol.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 15 2011

    Answer

    Thank you for your inquiry. I know that you said you're using nitrocellulose, and this protocol uses PVDF, but I hope you can adjust it accordingly. https://www.abcam.com/index.html?pageconfig=resource&rid=11456 I hope this information helps. Please contact us with any other questions.

    Read More

    Abcam Scientific Support

    Answered on Nov 15 2011

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