Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Molecular weight
      26 kDa

Specifications

Our Abpromise guarantee covers the use of ab43037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Immunoreactive with all sera of HIV1 infected individuals.
  • Applications

    ELISA

    Western blot

  • Purity
    > 95 % SDS-PAGE.
    Organic extraction > S-Sepharose > S-300.
  • Form
    Liquid
  • Additional notes

    Immunoreactive with all sera of HIV-1 infected individuals.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.20
    Constituents: 48% Urea, 0.605% Tris

General Info

  • Alternative names
    • CA
    • Capsid protein p24
    • HIV-1 Gag p24
    • HIV1gp1
    • Human immunodeficiency virus 1
    • Human immunodeficiency virus type 1 p24
    see all
  • Relevance
    HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • Cellular localization
    Membrane

References

This product has been referenced in:
  • Rosemary Bastian A  et al. Mechanism of multivalent nanoparticle encounter with HIV-1 for potency enhancement of peptide triazole virus inactivation. J Biol Chem 290:529-43 (2015). Read more (PubMed: 25371202) »
  • Hughes AJ & Herr AE Microfluidic Western blotting. Proc Natl Acad Sci U S A : (2012). SDS-PAGE . Read more (PubMed: 23223527) »

See all 2 Publications for this product

Publishing research using ab43037? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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What is the accession number of this protein and what is the amino acid range? What strain is it derived from?

Unfortunately we are unable to share the specific sequence but it is available in the literature. The protein is derived from group MN. These regions are highly conserved throughout all strains.

Has the activity of this protein been tested?

Thank you for contacting us.


The immune-biological activity of HIV p24 is derived from its linear AA sequences and has no secondary structure related activity. This protein has not been tested for functional activity.

I hope...

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The product is in 8M urea for the stock solution. My concern was, when the antigen is diluted to pg/mL level with a buffer without urea, is this antigen still stable or remain in the conformation that can be detected by ELISA?

You are correct, ab43037 is an antigen, not an antibody, and it is suitable to be coated on an ELISA plate from 100 ng to 10 ug. The dilution buffer that you use does not have to contain urea, as long as you are coating the wells within the range given...

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If this antibody is diluted further so the Urea concentration is low enough to not interfere with an ELISA, would the protein still be stable? I am concerned about the high Urea concentration interfering with the ELISA, so I want to make sure I dilute ...

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ab43037 works well in ELISA at nano to tens of micro gram levels. Thus, dilutions within that range should work sufficiently.

what concentration for ELISA?

Thank you for contacting us.

The laboratory provided the following ranges for ELISA. Using ab43037 protein use 100 ng to 10 ug to coat the plate, detection with ab81433 use at 1/5000-1/10000.

I hope this information is helpful to ...

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Hi,
I am interested in buy a p24 HIV recombinant protein to be used in a ELISPOT assay. Could you please tell me if you have any product that you known it is working in that assay and without inspecific response? We already buy one product from an...

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Thank you for contacting us.
I have checked some of the HIV1 p24 proteins available in our catalogue with my lab colleagues. Regarding their suitability for ELISPOT, I am afraid it hasn’t been tested in our lab, but due to their suitability in ELI...

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Do you have any ELISA kit for detection HIV-1 P24 antigen human IgG ?
Sample : Human serum
Thank you.

Thank you for contacting us.

Unfortunately, we do not have a kit for this purpose, but we have two products which may interest you:

https://www.abcam.com/index.html?datasheet=9071 (or use the following: https://www.abcam.com/index....

Read More

Thank you for your reply. it is very helpful.   The research I will carry out is to apply the magnetic detection echnique into HIV detection through sandwich assay.   From the information you provide, HIV1 p24 protein (ab73768) is the antigen I need, r...

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Thank you for your reply. Unfortunately, I would not recommend the protein ab73768. This is only a fragment of the HIV1 p24 that lacks large parts of the protein such as the N-terminal amino acids (1-76). If the antibodies you would like to test re...

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What is the difference between this four products? ab43037 (HIV1 p24 protein) ab73028 HIV1 p24 protein (His tag) ab43036 HTLV I p24 protein ab73768 HIV1 p24 protein I wantt to buy one HIV antigen, and two HIV mon...

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Thank you for contacting us. The differences are as follows: ab43036 HTLV I p24 protein: This protein is the full length sequence of HTLV I p24, i.e. an in E. coli derived recombinant protein. The Human T-lymphotropic virus Type I (HTLV-1) is not...

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I'm doing an ELISPOT-type assay with these products and am looking for a protocol.

Thank you for your inquiry. I know that you said you're using nitrocellulose, and this protocol uses PVDF, but I hope you can adjust it accordingly. https://www.abcam.com/index.html?pageconfig=resource&rid=11456 I hope this information helps. Please...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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