Description

  • Product name

    Recombinant HIV1 p24 protein
    See all HIV1 p24 proteins and peptides
  • Biological activity

    ab73768 is mmunoreactive with all sera of HIV1 infected individuals, and is an excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.
  • Purity

    > 95 % SDS-PAGE.
    ab73768 is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
  • Expression system

    Escherichia coli
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Amino acids

      77 to 436

Specifications

Our Abpromise guarantee covers the use of ab73768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

  • Form

    Liquid
  • Additional notes

    ab73768 is mmunoreactive with all sera of HIV1 infected individuals, and is an excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 48% Urea, 0.078% Beta mercaptoethanol, 0.316% Tris HCl

General Info

  • Alternative names

    • CA
    • Capsid protein p24
    • HIV-1 Gag p24
    • HIV1gp1
    • Human immunodeficiency virus 1
    • Human immunodeficiency virus type 1 p24
    see all
  • Relevance

    HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • Cellular localization

    Membrane

References

ab73768 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us.
I have checked some of the HIV1 p24 proteins available in our catalogue with my lab colleagues. Regarding their suitability for ELISPOT, I am afraid it hasn’t been tested in our lab, but due to their suitability in ELISA, they should also work in ELISPOT.
As an example, ab127888 is highly specific in ELISA, but ab73768 or ab43037 are also very likely to work in this assay.
If you would be interested, you could participate in our Antibody evaluation program, by which you test an antibody in a new species/application (such as any of the referred antibodies in ELISPOT), and share the results with us (submitting an Abreview), and you beneficiate from a free antibody.
If you want to read more about the Abreview system, please have a look at the next link:
https://www.abcam.com/abreviews
Please let me know if you would be interested in this program, and if you have any other doubts about it. I am happy to help, and clarify it further if necessary.
Have a very nice day!

Read More

Answer

Indeed, I can confirm that your type of experiments qualifies for the testing discount program. DISCOUNT CODES: ab73768: 100%ABR-E7TC5 ab8246: 100%ABR-E7TCE ab9071: 100%ABR-E7TCF Expiration date: 09 April 2012 I am very pleased to hear you would like to accept our offer and test these products in sELISA. These codes will give you 1 free primary antibody each (for ab8246 and ab9071) and 1 free protein (for ab73768) before the expiration date. To redeem this offer, please submit an Abreview for your sELISA experiments and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Question
Answer

You are welcome! Take all the time you need. I look forward to your reply whenever you are ready.

Read More

Answer

Thank you for your reply. Unfortunately, I would not recommend the protein ab73768. This is only a fragment of the HIV1 p24 that lacks large parts of the protein such as the N-terminal amino acids (1-76). If the antibodies you would like to test recognise an epitope within these missing parts (which we do not know as they have not been mapped), these antibodies would not be suitable for the proposed assay. Therefore, I would recommend to use the protein ab43037 which is the full-length HIV1 p24 and will be detected by the antibodies. Indeed, I can confirm that ab8246 and ab81433 are the same antibody clone, thus detecting the same epitope, and that ab81433 is conjugated to FITC which can be used in applications such as immunocytochemistry/immunofluorescence (ICC/IF). For your experiments it seems that you do not need the fluorochrome, so I would recommend ab8246. In summary, for the sELISA two antibodies that recognise different epitopes are required and the matching pair needs to be determined by the end user. To our knowledge, neither ab43037 nor ab8246/ab81433 nor ab9071 have yet been tested in sELISA. Therefore, I can offer a discount off a future purchase if you buy one or all of the products now, test it/them in sELISA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and which product you would like to test sELISA. I will then send a discount code. This code must be issued before purchasing the product(s) so please wait for my reply before ordering. 2. Purchase the product(s) either by phone, fax, or online (www.abcam.com). 3. Test it/them in sELISA. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody and protein ordered, respectively, and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the product(s) turn out to be unsuitable for sELISA, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting us. The differences are as follows: ab43036 HTLV I p24 protein: This protein is the full length sequence of HTLV I p24, i.e. an in E. coli derived recombinant protein. The Human T-lymphotropic virus Type I (HTLV-1) is not identical with HIV. When Human immunodeficiency virus (HIV), the virus that causes AIDS, was characterized, it was originally named HTLV-III. Therefore, this protein seems not suitable for your experiments. ab43037: This protein is the recombinant full length protein HIV1 p24, expressed in E.coli. This protein is guaranteed to work in Western blot and ELISA. ab73028: This protein is the recombinant full length HIV1 p24 from HxB2 strain purified from Pichia pastoris. It contains a 6x histidine fusion partner which for example facilitates purification. ab73768: This protein is only a recombinant fragment (amino acids 77-436) of the HIV1 p24 protein and is additionally fused to beta-galactosidase. If you could provide some more information on the type of assay you would like to perform and let us know whether you are interested in HIV-1 or HIV-2, I would be happy to make a specific recommendation. In case the assay is similar to a sandwich ELISA (sELISA), I can let you know that we have tested neither the proteins nor any antibodies for their suitability in sELISA, as this has to be tested for each pair individually. In this case, I would suggest to test ab43037 with the antibody clone [ND1] as in ab8246 or ab81433 and for example the monoclonal antibody ab9071. https://www.abcam.com/index.html?datasheet=8246 https://www.abcam.com/index.html?datasheet=8246. https://www.abcam.com/index.html?datasheet=81433 https://www.abcam.com/index.html?datasheet=81433. https://www.abcam.com/index.html?datasheet=9071 https://www.abcam.com/index.html?datasheet=9071. If you decide to go ahead and purchase one of these products, please contact our Scientific Support team prior to the purchase as you might be eligible for our testing discount program. Do not hesitate to contact us with more information to select the most suitable products for this experiment. I look forward to your reply.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up