Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE
Product nameRecombinant HIV1 p55 protein
Purity> 90 % SDS-PAGE.
Purified by several steps of chromatography
Expression systemEscherichia coli
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab63995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab63995 has been derived from infectious plasmid clone pNL4-3 and belongs to Group B of HIV-1.
Active as HIV-1 protease substrate
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 0.078% Beta mercaptoethanol, 0.316% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.29% Sodium chloride
- Gag polyprotein
RelevanceHIV1 (gag) p55 is a precursor protein of several proteins that form the core structure of AIDS virus, indispensable to its reproduction. This protein is digested by HIV1 protease, first into intermediate products p41 and p15. Then p41 is digested into matrix protein p17 and capsid protein p24. Protein p15 is further digested into nucleocapsid protein p7 and to p6 and p1; of unknown function. Nine genes are encoded within the HIV1 RNA genome. Three of these genes, gag, pol, and env, contain information needed to make the structural proteins for new virus particles.
SDS-PAGE analysis of Recombinant HIV1 p55 protein. As the substrate, Recombinant HIV1 p55 protein (1 μg, ab63995) was used in 20 μl reaction volume. The reaction was carried by incubating at 37℃ for 3 h and stopped by adding SDS-PAGE sample buffer. Lane 1: no protease,
Lane 2: 0.16 pg.
Lane 3: 1.6 pg.
Lane 4: 16 pg
Lane 5: 0.16 μg
Lane 6: 1.6 μg protease.
Note that two degradation bands are observed in the preparation of p55 substrate. In lane 4, p25 band is visible and in lane 5, p13 band is visible.
Polyacrylamide gel electrophoresis of ab63995.
The arrows show degradation products.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab63995 has not yet been referenced specifically in any publications.