Description

  • Product name

    Recombinant HIV1 Protease protein
    See all HIV1 Protease proteins and peptides
  • Biological activity

    Reaction buffer: 20 mM Tris-HCl (pH 6.8), 1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 10% Glycerol

  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Sequence

      PQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMSLPGRWKPKMIGGI GGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNF
    • Predicted molecular weight

      11 kDa
    • Amino acids

      501 to 599
    • Additional sequence information

      HIV1

Specifications

Our Abpromise guarantee covers the use of ab215523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    Western blot

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    When necessary freezing (with liquid or ethanol in dry-ice) and thawing (in water at room temperature with shaking) should be done rapidly.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C.

    pH: 6.50
    Constituents: 10% Glycerol, 0.24% Tris, 0.39% MES, 1.16% Sodium chloride, 0.03% EDTA, 0.02% DTT

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • HIV-1 protease

Images

  • ELISA of ab215523 using an anti-HIV protease antibody. Purified protease was spotted on wells.

  • SDS-PAGE analysis of ab215523.

  • Dot of ab215523. A anti-HIV-1 protease antibody was used at 1/2000 dilution, followed by a goat anti-rabbit IgG antibody conjugated with HRP secondary antibody at 1/5000 dilution.

  • SDS-PAGE analysis of proteolytic processing of HIV-1 Gag p55 proprotein by ab215523 in vitro.  Recombinant Gag p55 (1 μg) was used as the substrate in a 20 μl reaction volume. The reaction was carried by incubating at 37℃ for 3 h and stopped by adding SDS-PAGE sample buffer. 1; no protease, 2: 0.16 pg. 3; 1.6 pg. 4; 16 pg 5; 0.16 μg . 6; 1.6 μg ab215523. Note that two degradation bands are observed in the preparation of p55 substrate. In lane 4, p25 band is visible and in lane 5, p13 band is visible.

  • All lanes : Anti HIV1 protease at 1/2000 dilution

    Lane 1 : Recombinant HIV1 Protease protein (ab215523) at 0.001 µg
    Lane 2 : Extract of MT4 cells
    Lane 3 : Extract of MT4 cells infected with HIV-1 (LAI strain)

    Predicted band size: 10.8 kDa



    ab215523 as a control antigen for Western blot analysis.

References

ab215523 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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