Key features and details
- Expression system: Escherichia coli
- Active: Yes
- Suitable for: Functional Studies, WB, SDS-PAGE
Product nameRecombinant HIV1 Protease protein
See all HIV1 Protease proteins and peptides
Reaction buffer: 20 mM Tris-HCl (pH 6.8), 1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 10% Glycerol
Expression systemEscherichia coli
Protein lengthFull length protein
Predicted molecular weight11 kDa
Amino acids501 to 599
Additional sequence informationHIV1
Our Abpromise guarantee covers the use of ab215523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
When necessary freezing (with liquid or ethanol in dry-ice) and thawing (in water at room temperature with shaking) should be done rapidly.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C.
Constituents: 10% Glycerol (glycerin, glycerine), 0.24% Tris, 0.39% MES, 1.16% Sodium chloride, 0.03% EDTA, 0.02% DTT
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- HIV-1 protease
ELISA of ab215523 using an anti-HIV protease antibody. Purified protease was spotted on wells.
SDS-PAGE analysis of ab215523.
Dot of ab215523. A anti-HIV-1 protease antibody was used at 1/2000 dilution, followed by a goat anti-rabbit IgG antibody conjugated with HRP secondary antibody at 1/5000 dilution.
SDS-PAGE analysis of proteolytic processing of HIV-1 Gag p55 proprotein by ab215523 in vitro. Recombinant Gag p55 (1 μg) was used as the substrate in a 20 μl reaction volume. The reaction was carried by incubating at 37℃ for 3 h and stopped by adding SDS-PAGE sample buffer. 1; no protease, 2: 0.16 pg. 3; 1.6 pg. 4; 16 pg 5; 0.16 μg . 6; 1.6 μg ab215523. Note that two degradation bands are observed in the preparation of p55 substrate. In lane 4, p25 band is visible and in lane 5, p13 band is visible.
All lanes : Anti HIV1 protease at 1/2000 dilution
Lane 1 :
Recombinant HIV1 Protease protein (ab215523) at 0.001 µg
Lane 2 : Extract of MT4 cells
Lane 3 : Extract of MT4 cells infected with HIV-1 (LAI strain)
Predicted band size: 10.8 kDa
ab215523 as a control antigen for Western blot analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab215523 has not yet been referenced specifically in any publications.