Description

  • Product name

    Recombinant HIV1 Protease protein
    See all HIV1 Protease proteins and peptides
  • Biological activity

    One unit of HIV Protease hydrolyzes 1 picomole of a peptide (SQNYPIVQ) per minute at pH 4.7 at 25oC. 20-200ng is sufficient for an in vitro protease assay. HIV Protease can be applied in in vitro assay development and screening of protease inhibitors.

  • Purity

    > 95 % SDS-PAGE.
    Purified by an affinity chromatography in combination with FPLC chromatography
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Sequence

      PQITLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMSLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF
    • Predicted molecular weight

      12 kDa
    • Amino acids

      1 to 99
    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab84117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 3.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • HIV-1 protease

References

This product has been referenced in:

  • Boso G  et al. The nature of the N-terminal amino acid residue of HIV-1 RNase H is critical for the stability of reverse transcriptase in viral particles. J Virol 89:1286-97 (2015). Read more (PubMed: 25392207) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us yesterday and you interest in our products.

I have had a look into your query relating to whether the HIV protease proteins (ab113857, ab124313, ab124858 and ab84117) are all active. The title you have noticed where "Active" is included is down to an inconsistency in naming in our catalogue, it is not an indicator that ab113857 or ab124858 are not active. I am sorry for this confusion, I am going to try to have this amended.

Each one of the proteins have been shown to have enzyme activity through peptide cleavage assays. Details of these assays can be found on each of the individual datasheets of the products. This means that all of them should be suitable for your experiment if you are using a peptide sequence recognised by the protease. The experiment you are hoping to perform would be covered by the Abpromise guarantee. More information on this guarantee can be found from the following page:

https://www.abcam.com/abpromise

If you would like to try any of the proteins, I'd like to encourage you tosubmit anAbreview via the online product datasheet once you have used it in your assay. As a thank you you will be rewarded with Abpoints which can be used to redeem against Amazon vouchers or future purchases from us. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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Answer

Thank you for your patience. I apologize for the delay in getting back to you. I just received the reply from the supplying lab today. "For our experiments measuring the enzymatic activity, we have used both i) 100 mM MES (pH6), 200 mM NaCl, 0.1 mM EDTA and 1 mM DTT ii) 100 mM NaOAc buffer (pH5.5), 1 M NaCl, 1mM EDTA, 1 mM DTT, 0.02% Tween 20 Based on the literature, also 100 mM NaoAc, pH 4.7, 1 M NaCl, 1 mM EDTA, 1mM DTT gives a high activity for HIV protease and can be used." I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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