Product nameRecombinant HIV1 Reverse Transcriptase protein
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab63979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activitySpecific activity determined as 10,000 to 20,000 units/mg. Definition of activity: Activity of 1 nanomole intake of dTMP in 10 min is considered 1 unit with poly(rA) and oligo(dT) as template and primer at 37°C.
Additional notesab63979 has been derived from infectious plasmid clone pNL4-3 and belongs to Group B of HIV-1.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.1% Triton-X-100, 0.0475% Magnesium chloride, 0.078% Beta mercaptoethanol, 0.632% Tris HCl, 50% Glycerol, 0.29% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- HIV 1 Reverse transcriptase
- HIV1 RT
- Human Immunodeficiency virus 1
RelevanceHIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.