Overview

  • Product name
    Recombinant HIV1 Reverse Transcriptase protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli

Specifications

Our Abpromise guarantee covers the use of ab63979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity determined as 10,000 to 20,000 units/mg. Definition of activity: Activity of 1 nanomole intake of dTMP in 10 min is considered 1 unit with poly(rA) and oligo(dT) as template and primer at 37°C.
  • Applications

    SDS-PAGE

    Other

    Functional Studies

    Western blot

  • Form
    Liquid
  • Additional notes
    ab63979 has been derived from infectious plasmid clone pNL4-3 and belongs to Group B of HIV-1.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.30
    Constituents: 0.1% Triton-X-100, 0.0475% Magnesium chloride, 0.078% Beta mercaptoethanol, 0.632% Tris HCl, 50% Glycerol, 0.29% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • HIV 1 Reverse transcriptase
    • HIV1 RT
    • Human Immunodeficiency virus 1
    • p51 RT
    • p66 RT
    • Reverse transcriptase p51 subunit
    • Reverse transcriptase p66 subunit
    see all
  • Relevance
    HIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.

Images

  • Polyacrylamide gel electrophoresis of ab63979.

References

This product has been referenced in:
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Question
Answer



Yes there are 200 units/vial.

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Answer

Thank you for contacting us.
This can vary from lot to lot, but 1 vial of the current lot of HIV1 reverse transcriptase protein is 10 ug in 50 ul.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer



ab63979 HIV1 Reverse Transcriptase protein has been functionally tested as noted on the online datasheet, which reads

"Specific activity determined as 10,000 to 20,000 units/mg. Definition of activity: Activity of 1 nanomole intake of dTMP in 10 min is considered 1 unit with poly(rA) and oligo(dT) as template and primer at 37°C."

Further experimental details include:
"Definition of activity: Activity of intake of 1 nmole of dTMP in 10 min at 37℃ is considered as 1 unit using poly(rA) and oligo(dT) as template and primer.

Conditions of measurement: 50 mM Tris-HCl (pH 8.3), 10 mM MgCl2, 50mM KCl, 3 mM DTT, 0.1% Nonidet P-40, 20 ug/ml poly(rA)・oligo(dT)12-18, , 0.5 mM dTTP (with [3H] dTTP,1x105 cpm) , and 10-50 units/ml reverse transcriptase."

Unfortunately, further details regarding use of this protein as a reverse transcriptase are not available.

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Answer

Thank you for contacting Abcam regarding this product.
I have had the opportunity to speak with the lab regarding the specific activity of this product. Their response is as follows:
Definition of activity: Activity of intake of 1 nmole of dTMP in 10 min at 37℃ is considered as 1 unit using poly(rA) and oligo(dT) as template and primer.

Conditions of measurement: 50 mM Tris-HCl (pH 8.3), 10 mM MgCl2, 50mM KCl, 3 mM DTT, 0.1% Nonidet P-40, 20 ug/ml poly(rA)・oligo(dT)12-18, , 0.5 mM dTTP (with [3H] dTTP,1x105 cpm) , and 10-50 units/ml reverse transcriptase
These products are covered by our Abpromise guarantee. We provide scientific support, replacement or refund should this product not perform as indicated on the datasheet. More information on our Abpromise may be found at the following link:
https://www.abcam.com/index.html?pageconfig=abpromise
I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Question
Answer

Thank you for contacting Abcam.

I have heard back from the lab and they have told me that the protein concentration was measured by BCA method using assay kit from Pierce and the concentration was also confirmed by OD280. They measured the concentration of the whole protein rather just the active domain.

Please let me know if there is anything else I can help you with.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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