Product nameRecombinant HIV1 Reverse Transcriptase protein (Active)
Definition of activity: Activity of 1 nanomole intake of dTMP in 10 min is considered 1 unit with poly(rA) and oligo(dT) as template and primer at 37°C.
Purity> 90 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthFull length protein
Additional sequence informationComposed of two subunits whose molecular weights are 66 kD and 51 kD, same as the those of the enzyme purified from AIDS virus particles. (GenBank AAA44988.1)
Our Abpromise guarantee covers the use of ab63979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab63979 has been derived from infectious plasmid clone pNL4-3 and belongs to Group B of HIV-1.
1) It is extremely effective for screening new specific inhibitors for HIV virus as a drug for treating AIDS.
2) Generally, Gag and Env proteins are employed as antigens for detecting anti-HIV-1 antibody. However, by using this enzyme in combination as an antigen, the detection will be more sensitive.
3) Reverse transcriptases are used in the first step of RT-PCR reaction for converting RNA to DNA. The HIV-1 reverse transcriptase can also be applied for RT-PCR method.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Constituents: 0.1% Triton-X-100, 0.05% Magnesium chloride, 0.63% Tris HCl, 50% Glycerol, 0.29% Sodium chloride, 0.0154% DTT
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- HIV 1 Reverse transcriptase
- HIV1 RT
- Human Immunodeficiency virus 1
RelevanceHIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.
Polyacrylamide gel electrophoresis of ab63979.
All lanes : Anti-HIV1 Reverse Transcriptase antibody [39/4.12.2] (ab9066) at 1/2000 dilution
Lane 1 :
Recombinant HIV1 Reverse Transcriptase protein (Active) (ab63979) at 0.04 µg
Lane 2 :
Recombinant HIV1 Reverse Transcriptase protein (Active) (ab63979) at 0.02 µg
Lane 3 :
Recombinant HIV1 Reverse Transcriptase protein (Active) (ab63979) at 0.004 µg
Lane 4 :
Recombinant HIV1 Reverse Transcriptase protein (Active) (ab63979) at 0.002 µg
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Developed using the ECL technique.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab63979 has been referenced in 1 publication.
- Hughes AJ & Herr AE Microfluidic Western blotting. Proc Natl Acad Sci U S A : (2012). SDS-PAGE . PubMed: 23223527