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Customer called to ask how the intake of dTMP was determined when determining the specific activity of this product.
Specific activity determined as 10,000 to 20,000 units/mg. Definition of activity: Activity of 1 nanomole intake of dTMP in 10 min is considered 1 unit with poly(rA) and oligo(dT) as template and primer at 37°C.
Asked on Apr 27 2012
Thank you for contacting Abcam regarding this product.
I have had the opportunity to speak with the lab regarding the specific activity of this product. Their response is as follows:
Definition of activity: Activity of intake of 1 nmole of dTMP in 10 min at 37℃ is considered as 1 unit using poly(rA) and oligo(dT) as template and primer.
Conditions of measurement: 50 mM Tris-HCl (pH 8.3), 10 mM MgCl2, 50mM KCl, 3 mM DTT, 0.1% Nonidet P-40, 20 ug/ml poly(rA)・oligo(dT)12-18, , 0.5 mM dTTP (with [3H] dTTP,1x105 cpm) , and 10-50 units/ml reverse transcriptase
These products are covered by our Abpromise guarantee. We provide scientific support, replacement or refund should this product not perform as indicated on the datasheet. More information on our Abpromise may be found at the following link:
I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.
Answered on Apr 27 2012