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I am interested in HIV1 Reverse Transcriptase protein (ab63979) if it functional in vitro? Can it synthesize DNA from RNA template? What kind of RNA it may be and should it include some specific motifs? What you recomend to use for RNA template?\
Asked on Nov 26 2012
ab63979 HIV1 Reverse Transcriptase protein has been functionally tested as noted on the online datasheet, which reads
"Specific activity determined as 10,000 to 20,000 units/mg. Definition of activity: Activity of 1 nanomole intake of dTMP in 10 min is considered 1 unit with poly(rA) and oligo(dT) as template and primer at 37°C."
Further experimental details include:
"Definition of activity: Activity of intake of 1 nmole of dTMP in 10 min at 37℃ is considered as 1 unit using poly(rA) and oligo(dT) as template and primer.
Conditions of measurement: 50 mM Tris-HCl (pH 8.3), 10 mM MgCl2, 50mM KCl, 3 mM DTT, 0.1% Nonidet P-40, 20 ug/ml poly(rA)・oligo(dT)12-18, , 0.5 mM dTTP (with [3H] dTTP,1x105 cpm) , and 10-50 units/ml reverse transcriptase."
Unfortunately, further details regarding use of this protein as a reverse transcriptase are not available.
Answered on Nov 26 2012