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Our Abpromise guarantee covers the use of ab190123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Cytotoxicity can be detected in Human neutrophils when used in combination with Hlg B in a concentration range of 0.03-100 nM.
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 90% PBS, 10% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
SDS-PAGE and stain demonstrating 1 μg and 5 μg (lanes 1 and 2, respectively) of protein ab190123 under denaturing and reducing conditions.
Toxin Functionality: Human promyleocytic leukemia cell line HL60 was differentiated into neutrophils by treatment with DMSO. Neutrophils were incubated with serial dilutions of ab190123 and HlgC at equimolar concentration for 48 hours at 37°C with 5% CO2 and 95% humidity. Cellular viability was determined by adding XTT and incubation for additional 16 hours. OD’s were determined in the supernatants at 470nm. EC50 was found to be 8.92 nM of each toxin subunit.
ab190123 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"