Recombinant horse IL-5 protein (Active) (ab88009)

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Key features and details

  • Expression system: Yeast
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant horse IL-5 protein (Active)
    See all IL-5 proteins and peptides

  • Biological activity

    The biological activity of recombinant equine IL-5 was measured in a cell proliferation assay using the human TF-1 cell line. The ED50 for this effect is typically 20 - 30 ng/mL.

  • Purity

    > 95 % SDS-PAGE.
    Purified by ion-exchange chromatography.

  • Expression system

    Yeast

  • Accession

    O02699

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Equus

    • Sequence

      AVESPMNRLVAETLTLLSTHRTLLIGDGNLMIPTPEHKNHQLCIEEVFQG IDTLKNQTVQGDAVAKLFQNLSLIKGYIDLQKKKCGGERWRVKQFLDYLQ EFLGVINTEWTIEG

    • Predicted molecular weight

      13 kDa

    • Amino acids

      21 to 134

    • Additional sequence information

      This product is the mature full length protein from aa 21 - 134. The signal peptide is not included.

Specifications

Our Abpromise guarantee covers the use of ab88009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    Constituents: 90% PBS, 10% Trehalose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.

General Info

  • Alternative names

    • B-cell differentiation factor I
    • Colony stimulating factor
    • EDF
    • Eosinophil differentiation factor
    • IL-5
    • IL5
    • IL5_HUMAN
    • Interleukin 5
    • Interleukin 5 (colony stimulating factor, eosinophil)
    • Interleukin-5
    • T Cell Replacing Factor
    • T-cell replacing factor
    • TRF
    see all

  • Function

    Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.

  • Sequence similarities

    Belongs to the IL-5 family.

  • Cellular localization

    Secreted.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab88009? Please let us know so that we can cite the reference in this datasheet.

ab88009 has not yet been referenced specifically in any publications.

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