Recombinant horse IL-5 protein (Active) (ab88009)
Key features and details
- Expression system: Yeast
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant horse IL-5 protein (Active)
See all IL-5 proteins and peptides -
Biological activity
The biological activity of recombinant equine IL-5 was measured in a cell proliferation assay using the human TF-1 cell line. The ED50 for this effect is typically 20 - 30 ng/mL.
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Purity
> 95 % SDS-PAGE.
Purified by ion-exchange chromatography. -
Expression system
Yeast -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Equus -
Sequence
AVESPMNRLVAETLTLLSTHRTLLIGDGNLMIPTPEHKNHQLCIEEVFQG IDTLKNQTVQGDAVAKLFQNLSLIKGYIDLQKKKCGGERWRVKQFLDYLQ EFLGVINTEWTIEG -
Predicted molecular weight
13 kDa -
Amino acids
21 to 134 -
Additional sequence information
This product is the mature full length protein from aa 21 - 134. The signal peptide is not included.
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Specifications
Our Abpromise guarantee covers the use of ab88009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Constituents: 90% PBS, 10% Trehalose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.
General Info
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Alternative names
- B-cell differentiation factor I
- Colony stimulating factor
- EDF
see all -
Function
Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells. -
Sequence similarities
Belongs to the IL-5 family. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab88009 has not yet been referenced specifically in any publications.