Recombinant horse IL-1RA protein (Active) (ab243214)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, HPLC, SDS-PAGE
Description
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Product name
Recombinant horse IL-1RA protein (Active) -
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α- dependent proliferation of murine D10S cells is less than 3.0 μg/ml, corresponding to a specific activity of >333 IU/mg in the presence of 50 pg/ml rHuIL-1α.
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Purity
> 95 % SDS-PAGE.
>95% as determined by HPLC. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Horse -
Sequence
HPLGKRPCKMQAFRIWDVNQKTFYMRNNQLVAGYLQESNTKLQEKIDVVP IEPDALFLGLHGRKLCLACVKSGDEIRFQLEAVNITDLSKNKEENKRFTF IRSNSGPTTSFESAACPGWFLCTAQEADRPVSLTNKPKESFMVTKFYLQE DQ -
Predicted molecular weight
17 kDa -
Amino acids
26 to 177 -
Additional sequence information
Full-length mature chain lacking the signal peptide.
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Specifications
Our Abpromise guarantee covers the use of ab243214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
HPLC
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: PBS
0.2 µm filteredThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionBriefly centrifuge prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20 °C or below. Further dilutions should be made in appropriate buffered solutions.
General Info
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Alternative names
- ICIL1RA
- IL1F3
- IL1RA
see all -
Relevance
Interleukin 1 receptor antagonist inhibits the activities of interleukin 1 alpha and beta, and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other related cytokine genes form a cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is associated with increased risk of developing osteoporosis and gastric cancer. Four alternatively spliced transcript variants encoding differentt isoforms have been reported.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab243214 has not yet been referenced specifically in any publications.