Key features and details
- Expression system: Escherichia coli
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, HPLC, SDS-PAGE
Product nameRecombinant horse IL1 Receptor I/IL-1R-1 protein (Active)
See all IL1 Receptor I/IL-1R-1 proteins and peptides
Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α- dependent proliferation of murine D10S cells is less than 3.0 μg/ml, corresponding to a specific activity of >333 IU/mg in the presence of 50 pg/ml rHuIL-1α.
Endotoxin level< 1.000 Eu/µg
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceHPLGKRPCKMQAFRIWDVNQKTFYMRNNQLVAGYLQESNTKLQEKIDVVP IEPDALFLGLHGRKLCLACVKSGDEIRFQLEAVNITDLSKNKEENKRFTF IRSNSGPTTSFESAACPGWFLCTAQEADRPVSLTNKPKESFMVTKFYLQE DQ
Predicted molecular weight17 kDa
Amino acids26 to 177
Additional sequence informationFull-length mature chain lacking the signal peptide.
Our Abpromise guarantee covers the use of ab243214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
0.2 µm filtered
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionBriefly centrifuge prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20 °C or below. Further dilutions should be made in appropriate buffered solutions.
- Antigen CD121a
- CD121 antigen-like family member A
FunctionReceptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2.
Sequence similaritiesBelongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243214 has not yet been referenced specifically in any publications.