Question (63809) | Recombinant HPV16 L1 protein (ab119880)

Go to datasheet (ab119880)

Question

Kindly arrange to send us the COA & Method of Antigen Quantification for the below Abcam proteins

Answer



Below is a more detailed description of antigen quantification for these protein products:

The determination of the quantities of L1 proteins in each VLP preparation was based on the comparison with the known quantities of L1 (µL) and BSA proteins (µg) run in the same gel. Few quantities (1, 2, 3 µL per lane from the same VLP preparation) of HPV16 or HPV18 L1 VLPs were loaded on the SDS-PAGE gel next to BSA protein (0.1; 0,5; 1; 1,5, 2, 3 µg per lane). The quantities of L1 and BSA proteins loaded on the gel was determined by the densitometric scanning of the Coomassie brilliant blue stained protein bands fractionated by SDS-PAGE, using the ImageScanner III (GE Healthcare, Little Chalfont, UK ) device. Quantitative analysis was conducted with the ImageQuantTL software supplied with the instrument. At least three scans of each VLP preparation were obtained and average concentration was calculated.

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