Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 50.000 Eu/mg
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Product nameRecombinant HSP65 protein
See all HSP65 proteins and peptides
Biological activityThis product does not contain GroEL.
Purity> 90 % SDS-PAGE.
ab92416 is purified by Multi-step chromatography
Endotoxin level< 50.000 Eu/mg
Expression systemEscherichia coli
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab92416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesThis product does not contain GroEL.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.01999% Monobasic dihydrogen potassium phosphate, 0.11445% Dibasic monohydrogen sodium phosphate, 0.02025% Potassium chloride, 0.8004% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
RelevanceHSP65 is a member of the HSP60 family of heat shock proteins isolated from Mycobacterium bovis BCG. HSP65 from M. bovis is identical to that of M. tuberculosis, and similar to that of M. leprae, two highly pathogenic strains of mycobacterium. HSP65 is the immunodominant antigen during mycobacterial infection and vaccination, and has been linked to the development of autoimmune adjuvant arthritis in rats.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab92416 has been referenced in 1 publication.
- Kim BJ et al. Development of a Live Recombinant BCG Expressing Human Immunodeficiency Virus Type 1 (HIV-1) Gag Using a pMyong2 Vector System: Potential Use As a Novel HIV-1 Vaccine. Front Immunol 9:643 (2018). PubMed: 29636755