Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Additional sequence information
      The protein contains the HSV-1 gD immunodominant regions.

Specifications

Our Abpromise guarantee covers the use of ab43045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Immunoreactive with sera of HSV infected individuals, excellent antigen for detection of HSV with minimal specificity problems.
  • Applications

    ELISA

    Western blot

  • Purity
    > 95 % SDS-PAGE.
    Sepharose derived purification.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Constituents: 0.395% Tris HCl, 0.0292% EDTA, 50% Glycerol

General Info

  • Alternative names
    • GD
    • Glycoprotein D
    • Herpes simplex virus type 1 glycoprotein D
    • HSV1 glycoprotein D
  • Relevance
    Herpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low. During the latent phase the viral DNA is integrated into the genome of the host cell. Glycoprotein D (gD) has been implicated in binding to cellular receptors that facilitate virus penetration into cells. Herpes simplex virus type 1 (HSV-1) glycoprotein D (gD) is an essential component of the entry apparatus that is responsible for viral penetration and subsequent cell-cell spread.

References

ab43045 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Answer

Thank you for contacting Abcam.

For ab43045, we do not have the specific protocol information that validated this protein in WB and ELISA. We update the product datasheets with tested applications and species based on a variety of sources, whether it is our own lab work, specific references, or abreviews posted by researchers demonstrating successful use of the product in a particular application or species.

For ab48969 and ab48970, 2 micrograms of the protein were loaded in a SDS PAGE gel and a standard western blot protocol was followed. We do not have the specific protocol used to validate the protein in ELISA.

I'm sorry that this protocol information isn't more specific, but we do guarantee all 3 of these proteins to work in both western blot and ELISA as stated on the datasheet and will provide scientific support if there are any issues. If we cannot work with you to solve the issues, we will happily replace the product or refund your purchase within 6 months of purchase. Further details about our AbPromise guarantee are given through the link below:
https://www.abcam.com/index.html?pageconfig=resource&rid=10372&pid=10000&source=pagetrap

Our recommended western blot and ELISA protocols are given through the links below, and we also recommend optimizing the use of these proteins for both western blot and ELISA for each researcher's specific experimental conditions.
Western blotting: https://www.abcam.com/index.html?pageconfig=resource&rid=11375
ELISA (direct, indirect, fluorescent substrate, primary antibody): https://www.abcam.com/index.html?pageconfig=popular_protocols

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Merci pour votre requête et votre patience.

Comme xx est absent, j’ai fait suite à votre demande et j’ai contacté les laboratoires pour plus d’informations.

Je peux vous confirmer que le ab49027 est une protéine recombinante de E.coli contenant les séquences 1691-1710aa, 1712-1733aa, 1921-1940aa,du 1,2,3.5 génotypes (les régions immunodominants du HCV NS4). La séquence exacte est par contreune information propriétaire du laboratoire.

Malheureusement je ne peux pas vous donner plusd'informations sur lesséquences des autres protéines queceux notés sur les fiches techniques sous "protein description", car c'est des informations propriétaires et le laboratoire ne peux pas me les donner. Je suis très désolée pour ceci.

Est-ce que nous pouvons vous aider autrement?A quel but est-ce que vous avez besoin de ces séquences - est-ce que le laboratoire peux confirmer peux être l'utilité pour ceci?

Je suis désolée de ne pas pouvoir mieux vous aider en cette occasion. N'hésitez pas à me recontacter si nous pouvons vous aider autrement.

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Answer

Désolé pour le délai de ma réponse. Le tag GST est inclus dans les poids moléculaires des protéines suivantes : ab43045 HSV1 gD protein : 40,00 kDa ab43048 HSV1 gG Envelope protein : 45,50 kDa ab43050 VZV gE protein : 35,60 kDa ab43051 VZV ORF9 protein : 31,10 kDa ab43052 VZV ORF26 protein : 31,30 kDa ab67703 HSV2 gG Envelope Protein : 32,10 kDa ab67704 HHV8 RF8+ORF65 protein : 40,00 kDa (C-terminal immunodominant regions from ORF65 140-170 a.a. and N-terminal regions from ORF8 32-62 a.a.) Un erreur s'est glissée dans mon email précédent où j'ai indiqué que ab67704 avait un poids moléculaire de 32,10 kDa, le poids moléculaire de ab67704 est de 40 kDa. Désolé pour cette erreur. J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Answer

Merci de nous avoir contactés. Toutes protéines ci-dessous sont fusionnées avec le tag GST de 26 kDa Les poids moléculaires sont : ab43045 HSV1 gD protein : 40,00 kDa ab43048 HSV1 gG Envelope protein : 45,50 kDa ab43050 VZV gE protein : 35,60 kDa ab43051 VZV ORF9 protein : 31,10 kDa ab43052 VZV ORF26 protein : 31,30 kDa ab67703 HSV2 gG Envelope Protein : 32,10 kDa ab67704 HHV8 RF8+ORF65 protein : 32,10 kDa (C-terminal immunodominant regions from ORF65 140-170 a.a. and N-terminal regions from ORF8 32-62 a.a.) J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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