Recombinant HSV1 gD protein (ab43045)

Submit an Abreview Q&A (5)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Additional sequence information
      The protein contains the HSV-1 gD immunodominant regions.

Specifications

Our Abpromise guarantee covers the use of ab43045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Immunoreactive with sera of HSV infected individuals, excellent antigen for detection of HSV with minimal specificity problems.
  • Applications

    ELISA

    Western blot

  • Purity
    > 95 % SDS-PAGE.
    Sepharose derived purification.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Constituents: 0.395% Tris HCl, 0.0292% EDTA, 50% Glycerol

General Info

  • Alternative names
    • GD
    • Glycoprotein D
    • Herpes simplex virus type 1 glycoprotein D
    • HSV1 glycoprotein D
  • Relevance
    Herpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low. During the latent phase the viral DNA is integrated into the genome of the host cell. Glycoprotein D (gD) has been implicated in binding to cellular receptors that facilitate virus penetration into cells. Herpes simplex virus type 1 (HSV-1) glycoprotein D (gD) is an essential component of the entry apparatus that is responsible for viral penetration and subsequent cell-cell spread.

References

ab43045 has not yet been referenced specifically in any publications.

Publishing research using ab43045? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

5 reviews or Q&A

10
Votes: Highest First

I used this antibody and protein together in ELISA but they did not work. I would like a credit on the purchase.

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by pass...

Read More

Customer kindly inquiring about specific protocol used to validate ab43045, ab48969, ab48970 in WB and ELISA.

Thank you for contacting Abcam.

For ab43045, we do not have the specific protocol information that validated this protein in WB and ELISA. We update the product datasheets with tested applications and species based on a variety of sources, whe...

Read More

BOnjour,
je vous remercie pour votre réponse. Voici la liste des autres antigènes
dont j'aurais besoin de la séquence:
ab49027
ab43145
ab43137
ab43045
ab43048
ab43050
ab43052

Read More

Merci pour votre requête et votre patience.

Comme xx est absent, j’ai fait suite à votre demande et j’ai contacté les laboratoires pour plus d’informations.

Je peux vous confirmer que le ab49027...

Read More

Est-ce que les poids moléculaires que vous indiquez contiennent ou pas la GST ? Par exemple pour ab43045, le poids moléculaire total avec tag est-il de 40 kDa ou de 66 kDa ?

Désolé pour le délai de ma réponse. Le tag GST est inclus dans les poids moléculaires des protéines suivantes : ab43045 HSV1 gD protein : 40,00 kDa ab43048 HSV1 gG Envelope protein : 45,50 kDa ab43050 VZV gE protein : 35,60 kDa ab43051 VZV...

Read More

Bonjour, j'ai besoin de votre aide concernant ces antigènes : ab43045, ab43048, ab67703, ab648971, ab43050, ab43051, ab43052 et ab67704. Pouvez-vous me donner leurs poids moléculaires exacts et leur tag éventuels ?

Merci de nous avoir contactés. Toutes protéines ci-dessous sont fusionnées avec le tag GST de 26 kDa Les poids moléculaires sont : ab43045 HSV1 gD protein : 40,00 kDa ab43048 HSV1 gG Envelope protein : 45,50 kDa ab43050 VZV gE protein : 35,60 k...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up