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I’m trying to locate HSV monoclonal antibodies for use in western immunoblots. Specifically I need HSV-1 gC-1 (130kDa), HSV-2 gG-2 (92kDa) and HSV-2 140kDa glycoprotein. Your ABCAM on-line catalogue lists envelope proteins for both HSV-1 and -2 but does not seem to list their molecular weights. Is it possible to try some samples if you can get these monoclonals?
Could you please try monoclonals to HSV1 gG ab67739, HSV2 gG ab43047 and HSV2 gG ab67703. There does not seem to be any HSV1 gC. Could you ask if they can get some?
Could you please advise the molecular weight for the antibodies above, and if there is any plan for an HSV1 gc antibody to be produced?
Asked on Nov 20 2012
Thank you for contacting us.
The reference numbers ab67739, ab43047 and ab67703 given by your customer are not monoclonal antibodies but are the proteins. Can you check with them that this is indeed what they want?
Please let your customer know that we are not specialized in this particular field but I am happy to recommend antibodies.
We have ab6511 which is an anti-HSV1 gG Envelope Protein antibody [7F5] (https://www.abcam.com/6511).
We have several anti-HSV2 gG antibodies which I could also suggest.
Regarding an anti-HSV1 gC, we do have an Anti-HSV1 gC Envelope Protein antibody [3G9], ab6509 (https://www.abcam.com/ab6509) which detects a band at 95 kDa.
As for the molecular weights, they are not on the datasheets and I do not have images to refer to for all of these antibodies. Could you ask your customer where he got his predicted molecular weights from because I have had a thorough look and can only find lower molecular weight around 50 kDa for these proteins?
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447
Answered on Nov 20 2012