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Recombinant Human 14-3-3 alpha + beta protein (ab85273)

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SDS-PAGE - Recombinant Human 14-3-3 alpha + beta protein (ab85273)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Suitable for: SDS-PAGE, WB

    Description

    • Product name

      Recombinant Human 14-3-3 alpha + beta protein
      See all 14-3-3 alpha + beta proteins and peptides
    • Purity

      > 90 % SDS-PAGE.
      Purity was determined to be >90% by densitometry.
    • Expression system

      Escherichia coli
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human

    Associated products

    • Related Products

      • Anti-14-3-3 antibody [E58] (ab32377)
      • Anti-14-3-3 alpha + beta antibody [Y62] (ab32560)

    Specifications

    Our Abpromise guarantee covers the use of ab85273 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Western blot

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride

    General Info

    • Alternative names

      • 14 3 3 alpha
      • 14 3 3 protein beta/alpha
      • 14-3-3 protein beta/alpha
      • 1433B_HUMAN
      • Brain protein 14 3 3 beta isoform
      • GW128
      • HS 1
      • KCIP-1
      • KCIP1
      • N-terminally processed
      • Protein 1054
      • Protein kinase C inhibitor protein 1
      • YWHAA
      • YWHAB
      see all
    • Function

      Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
    • Sequence similarities

      Belongs to the 14-3-3 family.
    • Post-translational
      modifications

      The alpha, brain-specific form differs from the beta form in being phosphorylated.
      Isoform Short contains a N-acetylmethionine at position 1.
    • Cellular localization

      Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Target information above from: UniProt accession P31946 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human 14-3-3 alpha + beta protein (ab85273)
      SDS-PAGE - Recombinant Human 14-3-3 alpha + beta protein (ab85273)
      SDS-PAGE showing ab85273 at approximately 29kDa.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab85273? Please let us know so that we can cite the reference in this datasheet.

    ab85273 has not yet been referenced specifically in any publications.

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