Recombinant Human 14-3-3 alpha + beta protein (ab85273)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE, WB
Description
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Product name
Recombinant Human 14-3-3 alpha + beta protein
See all 14-3-3 alpha + beta proteins and peptides -
Purity
> 90 % SDS-PAGE.
Purity was determined to be >90% by densitometry. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab85273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride
General Info
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Alternative names
- 14 3 3 alpha
- 14 3 3 protein beta/alpha
- 14-3-3 protein beta/alpha
see all -
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. -
Sequence similarities
Belongs to the 14-3-3 family. -
Post-translational
modificationsThe alpha, brain-specific form differs from the beta form in being phosphorylated.
Isoform Short contains a N-acetylmethionine at position 1. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human 14-3-3 alpha + beta protein (ab85273)SDS-PAGE showing ab85273 at approximately 29kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab85273 has not yet been referenced specifically in any publications.
