Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE, WB
Product nameRecombinant Human 14-3-3 sigma/SFN protein
See all 14-3-3 sigma/SFN proteins and peptides
Purity> 95 % SDS-PAGE.
Purity was determined to be >95% by densitometry.
Expression systemEscherichia coli
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab85271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product was previously labelled as 14-3-3 sigma
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride
- 14 3 3 protein sigma
- 14-3-3 protein sigma
FunctionAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
p53-regulated inhibitor of G2/M progression.
Tissue specificityPresent mainly in tissues enriched in stratified squamous keratinizing epithelium.
Sequence similaritiesBelongs to the 14-3-3 family.
modificationsUbiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
Cellular localizationCytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab85271 has not yet been referenced specifically in any publications.