Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MREGPELSPD DPAGLLDLRQ GMFAQLVAQN VLLIDGPLSW YSDPGLAGVS LTGGLSYKED TKELVVAKAG VYYVFFQLEL RRVVAGEGSG SVSLALHLQP LRSAAGAAAL ALTVDLPPAS SEARNSAFGF QGRLLHLSAG QRLGVHLHTE ARARHAWQLT QGATVLGLFR VTPEIPAGLP SPRSE
    • Amino acids
      71 to 254

Specifications

Our Abpromise guarantee covers the use of ab50086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Endotoxin level
    < 0.100 Eu/µg
  • Purity
    > 95 % SDS-PAGE.
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

  • Reconstitution
    Reconsitute to 1mg/ml in distilled water.

General Info

  • Alternative names
    • 4 1BB L
    • 4 1BB ligand
    • 4 1BBL
    • 4-1BB ligand
    • 4-1BBL
    • Cd137l
    • Cd157l
    • Homolog of mouse 4 1BB L
    • Homolog of mouse 4 1BBL
    • ILA ligand (TNF related)
    • Ly63l
    • Receptor 4 1BB ligand
    • TNF superfamily member 9
    • TNFL9_HUMAN
    • Tnfsf9
    • TNLG5A
    • Tumor necrosis factor (ligand) superfamily member 9
    • Tumor necrosis factor ligand 5A
    • Tumor necrosis factor ligand superfamily member 9
    • Tumor necrosis factor superfamily member 9
    see all
  • Function
    Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.
  • Tissue specificity
    Expressed in brain, placenta, lung, skeletal muscle and kidney.
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Cellular localization
    Membrane.
  • Information by UniProt

References

This product has been referenced in:
  • Houtenbos I  et al. Leukemia-specific T-cell reactivity induced by leukemic dendritic cells is augmented by 4-1BB targeting. Clin Cancer Res 13:307-15 (2007). Read more (PubMed: 17170077) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer


I contacted our laboratory that used ab50086 to induce IL-8 production and they provided the following response:

"With reference to your query, the detailed protocol for the dose-dependent stimulation of IL-8 production by human PBMC which we employ to measure biological activity of ab50086 is as follows:
Human 4-IBB Ligand (4-IBBL) Stimulation of PBMC and Measurement of Induced Cytokines including TNF-a and IL-8
Stimulation of human PBMC by 4-1BBL is best observed using freshly isolated human PBMC. You can use RPMI alone or with added FCS or heat-inactivated human AB serum (1%) v/v. First add cells to the wells (0.5 mL of 5 X 106 /mL). Then soon thereafter, add (0.5 mL) increasing concentrations of 4-1BBL (l to 100 ng/mL, final concentration) to the cells. Place the plate in the incubator for 24 hours. Remove the supernatant carefully so that you do not gather cells. Alternatively, aspirate the well contents and centrifuge at 450 g for 15 minutes and measure IL-8 in the supernates. Some donors do not respond and the reason is unclear. Extending the time of incubation to 48 can also be tested but again it is donor variable.
Do not add 4-1BBL to the plastic well but only to the cells already in the Wells.
Measurement of other cytokines, including TNF-a is also possible but IL-8 is a reliable cytokine. Induction of cytokines should always include two controls: a negative control (no 4-1BBL) and a positive control. We recommend as the positive control adding 10 ng/mL of E. coli endotoxin (Sigma). If you culture the PBMC with 1% human AB serum, the negative control (no stimulant) will be higher for IL-8 production.
This assay can be done in a 96 flat bottom well plate with a maximum of 200 mL per well. Add 100 mL of cells then 100 mL of 4-1BBL. Again, the final concentration will be 1 to l00ng/mL in the wells. We have found that donor variability depends on concentration. For example, 1, 10, 20, 40 and 80 ng/mL final concentration would be the best dose-response to detecting a response.
The ED50 for this effect is 5-10 ng/ml. Please note results may vary with PBMC donors"

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Answer


An alternative name for the TNFSF9 protein is 4-1BB-L, which may help in literature searches for the effect of the protein on human PBMCs. I have included a pubmed link to the protein as a good starting point for a literature search:
http://www.ncbi.nlm.nih.gov/gene/8744

The TNFSF9 protein stimulates T lymphocytes, and this effect varies depending on the PBMC donor. An increase in interleukin production, including interleukin 8, appears to be a side effect of the T lymphocyte stimulation.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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