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Recombinant Human 5HT1A Receptor protein (ab132419)

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SDS-PAGE - Recombinant Human 5HT1A Receptor protein (ab132419)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: WB, SDS-PAGE, ELISA

    Description

    • Product name

      Recombinant Human 5HT1A Receptor protein

    • Expression system

      Wheat germ

    • Accession

      P08908

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MDVLSPGQGNNTTSPPAPFETGGNTTGISDVTVSYQVITSLLLGTLIFCA VLGNACVVAAIALERSLQNVANYLIGSLAVTDLMVSVLVLPMAALYQVLN KWTLGQVTCDLFIALDVLCCTSSILHLCAIALDRYWAITDPIDYVNKRTP RRAAALISLTWLIGFLISIPPMLGWRTPEDRSDPDACTISKDHGYTIYST FGAFYIPLLLMLVLYGRIFRAARFRIRKTVKKVEKTGADTRHGASPAPQP KKSVNGESGSRNWRLGVESKAGGALCANGAVRQGDDGAALEVIEVHRVGN SKEHLPLPSEAGPTPCAPASFERKNERNAEAKRKMALARERKTVKTLGII MGTFILCWLPFFIVALVLPFCESSCHMPTLLGAIINWLGYSNSLLNPVIY AYFNKDFQNAFKKIIKCKFCRQ

      • Predicted molecular weight

        73 kDa including tags

      • Amino acids

        1 to 422

      • Tags

        GST tag N-Terminus

    Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab132419 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      SDS-PAGE

      ELISA

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • 5 HT 1A
      • 5 HT receptor 1A
      • 5 HT1A
      • 5 HT1A receptor
      • 5 hydroxytryptamine (serotonin) receptor 1A
      • 5 hydroxytryptamine (serotonin) receptor 1A G protein coupled
      • 5 hydroxytryptamine receptor 1A
      • 5-HT-1A
      • 5-HT1A
      • 5-hydroxytryptamine receptor 1A
      • 5HT 1A
      • 5HT 1A receptor
      • 5HT receptor 1A
      • 5HT1a
      • 5HT1A_HUMAN
      • ADRB2RL1
      • ADRBRL1
      • G 21
      • G protein coupled receptor
      • G-21
      • Gpcr 18
      • Gpcr18
      • Guanine nucleotide binding regulatory protein-coupled receptor
      • Htr1a
      • PFMCD
      • Serotonin 1A receptor
      • Serotonin 5HT1A receptor
      • Serotonin receptor 1A
      see all

    • Function

      This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.

    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human 5HT1A Receptor protein (ab132419)
      SDS-PAGE - Recombinant Human 5HT1A Receptor protein (ab132419)
      12.5% SDS-PAGE analysis of ab132419, stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab132419? Please let us know so that we can cite the reference in this datasheet.

    ab132419 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Pre-absorption control for 5HT1A receptor antibody ab227165

     Poor
    Abreviews
    abreview image
    Application
    Functional Studies
    Material:
    Protein: ab132419
    Primary antibody: ab227165
    Tissue: frozen rat brain tissue sections
    Method: pre-absorption of
    staining of free-floating sections in well-plates
    Trial:
    Pre-absorption of the protein was accomplished by incubation of protein with primary antibody in carrier (dilution 1:1000) for 4 hours. Concentration of the primary antibody was 1 µg/ml and of the protein 5 µg/ml. The excess of protein was chosen on other pre-absorption control protocols. The pre-absorption was carried out prior to the previously established staining process with 3,3-Diamino benzidine. Carrier for the pre-absorption control and antibody consisted of goat serum and triton.
    Result:
    No pre-absorption of the primary antibody could be detected with this method. The staining of pre-absorption control and staining looked very similar.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 19 2021

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