Recombinant Human 68kDa Neurofilament/NF-L protein (His tag) (ab224840)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE
Description
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Product name
Recombinant Human 68kDa Neurofilament/NF-L protein (His tag) -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
SSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSL SVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRF ASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAE DATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADE AALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTK PDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAA KDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTI NKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLS FTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQ IEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEEAAEEEEAAKEE SEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD -
Predicted molecular weight
81 kDa including tags -
Amino acids
2 to 543 -
Tags
His tag N-Terminus -
Additional sequence information
10xHis-SUMO tag at the N-terminus and Myc tag at the C-terminus.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab224840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Mass Spectrometry
SDS-PAGE
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Mass spectrometry
LC-MS/MS -
Form
Liquid -
Additional notes
Previously labelled as 68kDa Neurofilament.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Constituents: 50% Glycerol (glycerin, glycerine), Tris buffer
General Info
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Alternative names
- 68 kDa neurofilament protein
- 68kDa Neurofilament
- 68kDa neurofilament protein
see all -
Function
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. -
Involvement in disease
Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. -
Sequence similarities
Belongs to the intermediate filament family. -
Domain
The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions. -
Post-translational
modificationsO-glycosylated.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
Images
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Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab224840 could indicate that this peptide derived from E.coli-expressed human Neurofilament protein.
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Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab224840 could indicate that this peptide derived from E.coli-expressed human Neurofilament protein.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab224840 has been referenced in 1 publication.
- Liu HC et al. Development of an assay of plasma neurofilament light chain utilizing immunomagnetic reduction technology. PLoS One 15:e0234519 (2020). PubMed: 32530970