Recombinant human AACT protein (ab182695)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human AACT protein
See all AACT proteins and peptides -
Biological activity
Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK(Dnp)-NH2.
The IC50 value is <6 nM.
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
CHPNSPLDEENLTQENQDRGTHVDLGLASANVDFAFSLYKQLVLKAPDKN VIFSPLSISTALAFLSLGAHNTTLTEILKGLKFNLTETSEAEIHQSFQHL LRTLNQSSDELQLSMGNAMFVKEQLSLLDRFTEDAKRLYGSEAFATDFQD SAAAKKLINDYVKNGTRGKITDLIKDLDSQTMMVLVNYIFFKAKWEMPFD PQDTHQSRFYLSKKKWVMVPMMSLHHLTIPYFRDEELSCTVVELKYTGNA SALFILPDQDKMEEVEAMLLPETLKRWRDSLEFREIGELYLPKFSISRDY N LNDILLQLGIEEAFTSKADLSGITGARNLAVSQVVHKAVLDVFEEGT EASAATAVKITLLSALVETRTIVRFNRPFLMIIVPTDTQNIFFMSKVTNP KQA -
Predicted molecular weight
23 kDa including tags -
Amino acids
23 to 423 -
Tags
His tag C-Terminus -
Additional sequence information
Mature form. AAH03559.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab182695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Reconstitute for long term storage.
pH: 7.4
Constituents: 0.88% Sodium chloride, 0.48% HEPES, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
General Info
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Alternative names
- SERPINA3
- AACT
- AACT_HUMAN
see all -
Function
Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. -
Tissue specificity
Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains. -
Involvement in disease
Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280]. -
Sequence similarities
Belongs to the serpin family. -
Domain
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
Cellular localization
Secreted. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab182695 has not yet been referenced specifically in any publications.