Recombinant human ABL1 protein (ab69810)

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Description

  • Product name

    Recombinant human ABL1 protein
    See all ABL1 proteins and peptides

  • Biological activity

    Specific activity: 871 nmol/min/mg

  • Expression system

    Baculovirus infected Sf9 cells

  • Accession

    P00519

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Predicted molecular weight

      135 kDa

    • Amino acids

      27 to 1130

    • Tags

      His tag N-Terminus

Associated products

Specifications

Our Abpromise guarantee covers the use of ab69810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

    ab204848 (ABL1 peptide) can be utilized as a substrate for assessing Kinase activity

    Previously labelled as c Abl.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Abelson murine leukemia viral oncogene homolog 1
    • Abelson tyrosine protein kinase 1
    • ABL
    • Abl 1
    • ABL proto oncogene 1 non receptor tyrosine kinase
    • ABL1
    • ABL1_HUMAN
    • bcr/abl
    • bcr/c abl oncogene protein
    • c ABL
    • c abl oncogene 1 non receptor tyrosine kinase
    • c abl oncogene 1 receptor tyrosine kinase
    • c ABL1
    • JTK7
    • p150
    • Proto oncogene tyrosine protein kinase ABL1
    • Proto-oncogene c-Abl
    • Tyrosine-protein kinase ABL1
    • v abl
    • v abl Abelson murine leukemia viral oncogene homolog 1
    see all

  • Function

    Protein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.

  • Tissue specificity

    Widely expressed.

  • Involvement in disease

    Note=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.

  • Post-translational
    modifications

    Phosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation.
    Isoform IB is myristoylated on Gly-2.

  • Cellular localization

    Cytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human ABL1 protein (ab69810)
    Functional Studies - Recombinant human ABL1 protein (ab69810)
    The specific activity of ABL1 (ab69810) was determined to be 765.8 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human ABL1 protein (ab69810)
    SDS-PAGE - Recombinant human ABL1 protein (ab69810)
    SDS PAGE analysis of ab69810
  • SDS-PAGE - Recombinant human ABL1 protein (ab69810)
    SDS-PAGE - Recombinant human ABL1 protein (ab69810)
    SDS PAGE analysis of ab69810
  • Functional Studies - Recombinant human ABL1 protein (ab69810)
    Functional Studies - Recombinant human ABL1 protein (ab69810)
    Kinase activity assay of ab69810. The specific activity of c-Abl was determined to be 871 nmol/min/mg.
  • SDS-PAGE - Recombinant human ABL1 protein (ab69810)
    SDS-PAGE - Recombinant human ABL1 protein (ab69810)
    SDS-PAGE of ab69810. Molecular weight 135kDa.

References

This product has been referenced in:

  • Campbell H  et al. SHP-2 is activated in response to force on E-cadherin and dephosphorylates vinculin Y822. J Cell Sci 131:N/A (2018). Read more (PubMed: 30478196) »
See 1 Publication for this product

Publishing research using ab69810? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Question

We are wondering what are the residues of Abl that are part of this recombinant protein. The spec sheet indicates that it if from residues "0 to 0"? The blot indicates a molecular weight around 130 Kda which would correspond to the full length protein. It is also supposed to be active. Does this mean that is a mutant form of Abl.

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Answer

This protein corresponds to amino acids 27-1130 of the human protein. It also includes 9 amino acids of a His tag fused to the N-terminus.

This protein should therefore been seen at ˜135 kDa on a blot. It is not a mutated version of the protein, and therefore it needs to be activated. We have performed kinase activity assays of ab69810. The specific activity of c-Abl was determined to be 780 nmol/min/mg for our current lot in stock: GR131302-1 .

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Question

The customer asks if they can replace MOPS with HEPES and if so, is it supposed to be at the same concentration (25mM)?

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Answer

Yes, HEPES pH 7.2 can be substituted for MOPS in the kinase assay buffer, at the same concentration, 25mM.

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Question

Please advise for the buffer recommended for Functional Studies.

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Answer

Please find attached the functional assay (kinase activity) for ab69810, which includes buffer recommendations.

Please let me know if you have any questions.

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Question

Dear All,

Please confirm the price of items AB-ab84546 c Abl protein (Active), 5 ug and AB-ab69810 c Abl protein (Active), 10 ug.

The items are in different sizes but have the same description and same price (275.6$).

Is it correct?

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Answer

Thank you for your message.

I apologize for any confusion. I have checked with our products department who have confirmed that this was a duplicate in our catalog, ab84546 and ab69810 are both the same.

We have therefore removed ab84546 c Abl protein (Active)from the catalog. Both the 5 ug and 10 ug sizes are now available as ab69810.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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