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We are wondering what are the residues of Abl that are part of this recombinant protein. The spec sheet indicates that it if from residues "0 to 0"? The blot indicates a molecular weight around 130 Kda which would correspond to the full length protein. It is also supposed to be active. Does this mean that is a mutant form of Abl.
Asked on Dec 02 2013
This protein corresponds to amino acids 27-1130 of the human protein. It also includes 9 amino acids of a His tag fused to the N-terminus.
This protein should therefore been seen at ˜135 kDa on a blot. It is not a mutated version of the protein, and therefore it needs to be activated. We have performed kinase activity assays of ab69810. The specific activity of c-Abl was determined to be 780 nmol/min/mg for our current lot in stock: GR131302-1 .
Elisa ThomasAbcam Scientific Support
Answered on Dec 02 2013