Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies
Product nameRecombinant human ABL2 protein
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
Amino acids38 to 1167
TagsHis tag N-Terminus
Our Abpromise guarantee covers the use of ab51259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab204848 (c Abl peptide) can be utilized as a substrate for assessing Kinase activity
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: 1.02% Imidazole
Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00308% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Abelson murine leukemia viral oncogene homolog 2
- Abelson related gene protein
- Abelson tyrosine-protein kinase 2
FunctionNon-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
DomainContains two distinct classes of F-actin-binding domains. Although both can bind F-actin, the 2 are required to bundle actin filaments.
modificationsPhosphorylated at Tyr-261 by ABL1 in response to oxidative stress. Phosphorylated by PDGFRB.
Polyubiquitinated. Polyubiquitination of ABL2 leads to degradation.
Cellular localizationCytoplasm, cytoskeleton.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab51259 has not yet been referenced specifically in any publications.