Description

  • Product name

    Recombinant Human ACAT1 protein
  • Purity

    > 95 % SDS-PAGE.
    ab137175 is purified using conventional chromatography techniques.
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSVSKPTLK EVVIVSATRT PIGSFLGSLS LLPATKLGSI AIQGAIEKAG IPKEEVKEAY MGNVLQGGEG QAPTRQAVLG AGLPISTPCT TINKVCASGM KAIMMASQSL MCGHQDVMVA GGMESMSNVP YVMNRGSTPY GGVKLEDLIV KDGLTDVYNK IHMGSCAENT AKKLNIARNE QDAYAINSYT RSKAAWEAGK FGNEVIPVTV TVKGQPDVVV KEDEEYKRVD FSKVPKLKTV FQKENGTVTA ANASTLNDGA AALVLMTADA AKRLNVTPLA RIVAFADAAV EPIDFPIAPV YAASMVLKDV GLKKEDIAMW EVNEAFSLVV LANIKMLEID PQKVNINGGA VSLGHPIGMS GARIVGHLTH ALKQGEYGLA SICNGGGGAS AMLIQKL
    • Predicted molecular weight

      44 kDa including tags
    • Amino acids

      34 to 427
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab137175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry

    MALDI-TOF
  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.02% DTT, 0.32% Tris HCl, 10% Glycerol, 0.58% Sodium chloride

General Info

  • Alternative names

    • ACAT
    • ACAT 1
    • acat1
    • Acetoacetyl CoA thiolase
    • acetoacetyl Coenzyme A thiolase
    • Acetoacetyl-CoA thiolase
    • Acetyl CoA acetyltransferase, mitochondrial
    • Acetyl Coenzyme A acetyltransferase 1
    • Acetyl-CoA acetyltransferase
    • acetyl-coa acetyltransferase precursor, mitochondrial
    • Acetyl-CoA thiolase, mitochondrial
    • acetyl-Coenzyme A acetyltransferase 1
    • MAT
    • mitochondrial
    • mitochondrial acetoacetyl-CoA thiolase
    • RATACAL
    • T2
    • testicular tissue protein Li 198
    • THIL
    • THIL_HUMAN
    see all
  • Function

    Plays a major role in ketone body metabolism.
  • Involvement in disease

    Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD) [MIM:203750]; also known as alpha-methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype.
  • Sequence similarities

    Belongs to the thiolase family.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of ab137175 (3µg)

References

ab137175 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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