Recombinant Human ACE2 protein (ab151852)

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Western blot - Recombinant Human ACE2 protein (ab151852)

    Key features and details

    • Expression system: HEK 293 cells
    • Purity: > 95% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Tags: His tag C-Terminus
    • Suitable for: SDS-PAGE, HPLC

    Description

    • Product name

      Recombinant Human ACE2 protein
      See all ACE2 proteins and peptides

    • Purity

      > 95 % SDS-PAGE.

    • Endotoxin level

      < 1.000 Eu/µg

    • Expression system

      HEK 293 cells

    • Accession

      Q9BYF1

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGD KWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLN TILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWES WRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDY SRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLL GDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVS VGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMD DFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKH LKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGE IPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYT RTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWT LALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQS IKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGE EDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRL NDNSLEFLGIQPTLGPPNQPPVSVDHHHHHH

      • Predicted molecular weight

        85 kDa including tags

      • Amino acids

        18 to 740

      • Tags

        His tag C-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab151852 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      HPLC

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.4
      Constituents: 0.001% Zinc chloride, 0.32% Tris HCl, 10% Glycerol, 0.88% Sodium chloride

    General Info

    • Alternative names

      • ACE 2
      • ACE related carboxypeptidase
      • ACE-related carboxypeptidase
      • ACE2
      • ACE2_HUMAN
      • ACEH
      • Angiotensin converting enzyme 2
      • Angiotensin converting enzyme homolog
      • Angiotensin converting enzyme like protein
      • Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2
      • Angiotensin I converting enzyme 2
      • Angiotensin-converting enzyme homolog
      • DKFZP434A014
      • EC 3.4.17
      • metalloprotease MPROT 15
      • Metalloprotease MPROT15
      • OTTHUMP00000022963
      • Processed angiotensin-converting enzyme 2
      see all

    • Function

      Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.

    • Tissue specificity

      Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.

    • Sequence similarities

      Belongs to the peptidase M2 family.

    • Post-translational
      modifications

      N-glycosylation on Asn-90 may limit SARS infectivity.

    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt

    Images

    • Western blot - Recombinant Human ACE2 protein (ab151852)
      Western blot - Recombinant Human ACE2 protein (ab151852)
      Recombinant Human ACE2 protein (ab151852) + Human ACE-2 Protein (C-His), reducing condition

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (4)

    Publishing research using ab151852? Please let us know so that we can cite the reference in this datasheet.

    ab151852 has been referenced in 4 publications.

    • Zhang Q  et al. Angiotensin-converting Enzyme 2-containing Small Extracellular Vesicles and Exomeres Bind the Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein. Gastroenterology 160:958-961.e3 (2021). PubMed: 33022277
    • D'Onofrio N  et al. Glycated ACE2 receptor in diabetes: open door for SARS-COV-2 entry in cardiomyocyte. Cardiovasc Diabetol 20:99 (2021). PubMed: 33962629
    • Hudák A  et al. Contribution of Syndecans to the Cellular Entry of SARS-CoV-2. Int J Mol Sci 22:N/A (2021). PubMed: 34069441
    • Casciola-Rosen L  et al. IgM autoantibodies recognizing ACE2 are associated with severe COVID-19. medRxiv N/A:N/A (2020). PubMed: 33083808

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