Recombinant Human Acid Phosphatase 2 protein (denatured) (ab202151)
Key features and details
- Expression system: Escherichia coli
- Purity: > 85% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human Acid Phosphatase 2 protein (denatured) -
Purity
> 85 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMGSRSLRFVTLLYRHGDRSPVKTYPKDPYQ EEEWPQGFGQLTKEGMLQHWELGQALRQRYHGFLNTSYHRQEVYVRSTDF DRTLMSAEANLAGLFPPNGMQRFNPNISWQPIPVHTVPITEDRLLKFPLG PCPRYEQLQNETRQTPEYQNESSRNAQFLDMVANETGLTDLTLETVWNVY DTLFCEQTHGLRLPPWASPQTMQRLSRLKDFSFRFLFGIYQQAEKARLQG GVLLAQIRKNLTLMATTSQLPKLLVYSAHDTTLVALQMALDVYNGEQAPY ASCHIFELYQEDSGNFSVEMYFRNESDKAPWPLSLPGCPHRCPLQDFLRL TEPVVPKDWQQECQLASGPADTE -
Predicted molecular weight
43 kDa including tags -
Amino acids
31 to 380 -
Tags
His tag N-Terminus -
Additional sequence information
Lumenal domain (NP_001601).
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Description
Recombinant Human Acid Phosphatase 2 protein
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab202151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl
General Info
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Alternative names
- Acid phosphatase 2 lysosomal
- ACP 2
- ACP2
see all -
Involvement in disease
Defects in ACP2 are a cause of acid phosphatase deficiency (ACPHD) [MIM:200950]. The clinical features are intermittent vomiting, hypotonia, lethargy, opisthotonos, terminal bleeding, and death in early infancy. Lysosomal acid phosphatase is deficient in cultured fibroblasts and multiple tissues. -
Sequence similarities
Belongs to the histidine acid phosphatase family. -
Post-translational
modificationsThe membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen.
N-glycosylated. The intermediates formed during enzymatic deglycosylation suggest that all eight predicted N-glycosylation sites are used. -
Cellular localization
Lysosome membrane. Lysosome lumen. The soluble form arises by proteolytic processing of the membrane-bound form. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab202151 has not yet been referenced specifically in any publications.